Avian Flavivirus Infection of Monocytes/Macrophages by Extensive Subversion of Host Antiviral Innate Immune Responses.
Animals
Chickens
/ virology
China
Ducks
/ virology
Female
Flavivirus
/ immunology
Flavivirus Infections
/ immunology
Host Specificity
/ genetics
Host-Pathogen Interactions
/ genetics
Immunity, Innate
/ drug effects
Macrophages
/ drug effects
Monocytes
/ drug effects
Poultry Diseases
/ virology
Virus Replication
/ drug effects
: flavivirus
innate immunity
monocytes/macrophages
virus-host interactions
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
15 11 2019
15 11 2019
Historique:
received:
12
06
2019
accepted:
19
08
2019
pubmed:
30
8
2019
medline:
21
7
2020
entrez:
30
8
2019
Statut:
epublish
Résumé
Avian Tembusu virus (TMUV) is a newly emerging avian pathogenic flavivirus in China and Southeast Asia with features of rapid spread, an expanding host range, and cross-species transmission. The mechanisms of its infection and pathogenesis remain largely unclear. Here, we investigated the tropism of this arbovirus in peripheral blood mononuclear cells of specific-pathogen-free (SPF) ducks and SPF chickens and identified monocytes/macrophages as the key targets of TMUV infection. In vivo studies in SPF ducks and SPF chickens with monocyte/macrophage clearance demonstrated that the infection of monocytes/macrophages was crucial for viral replication, transmission, and pathogenesis. Further genome-wide transcriptome analyses of TMUV-infected chicken macrophages revealed that host antiviral innate immune barriers were the major targets of TMUV in macrophages. Despite the activation of major pattern recognition receptor signaling, the inductions of alpha interferon (IFN-α) and IFN-β were blocked by TMUV infection on transcription and translation levels, respectively. Meanwhile, TMUV inhibited host redox responses by repressing the transcription of genes encoding NADPH oxidase subunits and promoting Nrf2-mediated antioxidant responses. The recovery of either of the above-mentioned innate immune barriers was sufficient to suppress TMUV infection. Collectively, we identify an essential step of TMUV infection and reveal extensive subversion of host antiviral innate immune responses.
Identifiants
pubmed: 31462573
pii: JVI.00978-19
doi: 10.1128/JVI.00978-19
pmc: PMC6819932
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2019 American Society for Microbiology.
Références
Emerg Infect Dis. 2001 Jul-Aug;7(4):751-3
pubmed: 11585545
J Gen Virol. 2017 Oct;98(10):2413-2420
pubmed: 28874226
J Gen Virol. 2017 Oct;98(10):2401-2412
pubmed: 28884667
PLoS One. 2017 Aug 1;12(8):e0181801
pubmed: 28763472
PLoS One. 2011 Mar 24;6(3):e18106
pubmed: 21455312
Int J Mol Sci. 2010 Sep 29;11(10):3769-82
pubmed: 21152300
Cell Host Microbe. 2016 Feb 10;19(2):181-93
pubmed: 26867177
Viruses. 2019 Mar 12;11(3):
pubmed: 30871003
Virol J. 2013 Aug 14;10:260
pubmed: 23941427
J Virol. 2016 Jun 24;90(14):6538-6548
pubmed: 27147750
Cell Res. 2011 Jan;21(1):103-15
pubmed: 21187859
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
Infect Genet Evol. 2015 Oct;35:27-33
pubmed: 26205688
Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10682-7
pubmed: 16030144
Annu Rev Immunol. 2008;26:421-52
pubmed: 18303997
Immunol Cell Biol. 2007 Aug-Sep;85(6):435-45
pubmed: 17667934
J Virol. 2017 Feb 28;91(6):
pubmed: 28031363
Proc Natl Acad Sci U S A. 2005 Jan 4;102(1):244-9
pubmed: 15611470
Transbound Emerg Dis. 2015 Apr;62(2):209-16
pubmed: 23711093
J Virol. 2019 Feb 5;93(4):
pubmed: 30518647
Emerg Infect Dis. 2011 Oct;17(10):1873-5
pubmed: 22000358
Biochem Biophys Res Commun. 2017 Oct 28;492(4):533-540
pubmed: 28167278
J Med Entomol. 1994 Nov;31(6):934-8
pubmed: 7815413
Nucleic Acids Res. 2013 May;41(9):4743-54
pubmed: 23519614
Cell Host Microbe. 2016 Jul 13;20(1):83-90
pubmed: 27247001
Science. 2010 Feb 5;327(5966):656-61
pubmed: 20133564
Influenza Other Respir Viruses. 2009 Jul;3(4):121-8
pubmed: 19627369
PLoS One. 2016 Feb 26;11(2):e0150568
pubmed: 26918620
Nat Rev Immunol. 2011 Oct 14;11(11):723-37
pubmed: 21997792
Clin Vaccine Immunol. 2014 Aug;21(8):1046-53
pubmed: 24872514
Viral Immunol. 2015 Nov;28(9):478-88
pubmed: 26301315
J Exp Med. 1977 Jul 1;146(1):201-17
pubmed: 406347
Front Immunol. 2017 Jan 31;8:49
pubmed: 28197148
Vet Res. 2016 Aug 05;47(1):75
pubmed: 27494935
J Gen Virol. 2013 Jun;94(Pt 6):1189-1194
pubmed: 23426356
J Gen Virol. 2003 Oct;84(Pt 10):2635-45
pubmed: 13679597
Vet Res. 2016 Jul 13;47(1):70
pubmed: 27412035
EMBO J. 2017 Mar 1;36(5):604-616
pubmed: 28122869
Curr Opin Immunol. 2006 Feb;18(1):49-53
pubmed: 16338128
J Gen Virol. 2019 Feb;100(2):119-132
pubmed: 30628886
Virology. 2012 Apr 10;425(2):82-94
pubmed: 22305622
J Virol. 2018 Aug 29;92(18):
pubmed: 29950417
Vet Immunol Immunopathol. 2013 Oct 1;155(4):270-5
pubmed: 24034933
Biochem Biophys Res Commun. 2017 Oct 28;492(4):587-596
pubmed: 28576494
Am J Reprod Immunol. 2017 Feb;77(2):
pubmed: 27966815
J Biomed Sci. 2018 Nov 8;25(1):77
pubmed: 30409217
J Virol. 2018 Aug 16;92(17):
pubmed: 29899104
Biomed Res Int. 2014;2014:315470
pubmed: 25101271
Vet Microbiol. 2019 Feb;229:138-146
pubmed: 30642589
Virus Res. 2013 Sep;176(1-2):216-22
pubmed: 23830999
PLoS One. 2015 Jul 17;10(7):e0133450
pubmed: 26186542
PLoS Pathog. 2014 Dec 18;10(12):e1004566
pubmed: 25521078
Cell Stem Cell. 2016 Dec 1;19(6):752-767
pubmed: 27641306
Emerg Infect Dis. 2011 Aug;17(8):1498-501
pubmed: 21801633
Virol J. 2016 Jun 14;13:101
pubmed: 27296632
PLoS Pathog. 2010 Jun 03;6(6):e1000930
pubmed: 20532218
Front Microbiol. 2016 Feb 19;7:190
pubmed: 26925054
Vet Immunol Immunopathol. 2012 Aug 15;148(3-4):353-8
pubmed: 22835899
Curr Protoc Mol Biol. 2010 Jan;Chapter 19:Unit 19.10.1-21
pubmed: 20069535
Acta Neuropathol. 2017 Oct;134(4):655-666
pubmed: 28623559
Nat Rev Immunol. 2008 Dec;8(12):911-22
pubmed: 18989317