Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.
G-quadruplex peroxidase
NASBA
Pathogen detection
colorimetric test
deoxyribozyme cascade
isothermal amplification
Journal
Sensors and actuators. B, Chemical
ISSN: 0925-4005
Titre abrégé: Sens Actuators B Chem
Pays: Switzerland
ID NLM: 101149755
Informations de publication
Date de publication:
01 Mar 2019
01 Mar 2019
Historique:
entrez:
30
8
2019
pubmed:
30
8
2019
medline:
30
8
2019
Statut:
ppublish
Résumé
A colorimetric nucleic acid based test for label-free pathogen detection has been developed and used for the detection of the Zika virus. The test relies on nucleic acid sequence-based amplification (NASBA) of a viral RNA followed by interrogation of the amplicon by a cascade of deoxyribozymes constituting a visual split deoxyribozyme (vsDz) probe. The probe consists of a split phosphodiesterase deoxyribozyme, which forms its catalytic core upon binding to a specific amplicon fragment. The catalytically active complex recognizes and cleaves an inhibited peroxidase-like deoxyribozyme (PDz), thereby activating it. Active PDz catalyzes hydrogen peroxide-mediated oxidation of a colorless substrate into a colored product, thereby generating a visible signal. Viral RNA (10
Identifiants
pubmed: 31462856
doi: 10.1016/j.snb.2018.11.147
pmc: PMC6713451
mid: NIHMS1515871
doi:
Types de publication
Journal Article
Langues
eng
Pagination
945-951Subventions
Organisme : NIAID NIH HHS
ID : R01 AI110692
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI123876
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI151537
Pays : United States
Déclaration de conflit d'intérêts
Notes The authors declare no competing financial interest.
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