Mesenchymal stem cell therapies for intervertebral disc degeneration: Consideration of the degenerate niche.
degeneration
hydrogel
intervertebral disc
regeneration
Journal
JOR spine
ISSN: 2572-1143
Titre abrégé: JOR Spine
Pays: United States
ID NLM: 101722350
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
11
06
2018
accepted:
08
05
2019
entrez:
30
8
2019
pubmed:
30
8
2019
medline:
30
8
2019
Statut:
epublish
Résumé
We have previously reported a synthetic Laponite crosslinked poly N-isopropylacrylamide-co-N, N'-dimethylacrylamide (NPgel) hydrogel, which induces nucleus pulposus (NP) cell differentiation of human mesenchymal stem cells (hMSCs) without the need for additional growth factors. Furthermore NP gel supports integration following injection into the disc and restores mechanical function to the disc. However, translation of this treatment strategy into clinical application is dependent on the survival and differentiation of hMSC to the correct cell phenotype within the degenerate intervertebral disc (IVD). Here, we investigated the viability and differentiation of hMSCs within NP gel within a catabolic microenvironment. hMSCs were encapsulated in NPgel and cultured for 4 weeks under hypoxia (5% O
Identifiants
pubmed: 31463465
doi: 10.1002/jsp2.1055
pii: JSP21055
pmc: PMC6686825
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e1055Subventions
Organisme : Medical Research Council
ID : MR/P026796/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
C.L.M. and C.S. are named inventors on a patent for the L‐pNIPAM hydrogel described here.
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