Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells.
Cervical cancer
Network
RNA
Sequencing
Transcriptome
miR-214
Journal
Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
05
07
2019
revised:
19
08
2019
accepted:
21
08
2019
pubmed:
30
8
2019
medline:
5
1
2021
entrez:
30
8
2019
Statut:
ppublish
Résumé
In this study, we investigate the effect of one such micro RNA, miR-214 which is frequently down-regulated in cervical cancer. In this study, we either CRISPR knocked out or overexpressed miR-214 in cervical cancer cells and analyzed the global mRNA expression by Next Generation Sequencing (NGS) It was observed that a total of 108 genes were upregulated and 178 downregulated between the samples, above and below the baseline respectively. Gene Ontology and KEGG pathway analysis reveal distinct biological processes and pathways. Analysis of gene regulatory networks also gave different network patterns in the two samples. We confirmed the RNA sequencing data for 10 genes; IFIF27, SMAD3, COX11, TP53INP1, ABL2, FGF8, TNFAIP3, NRG1, SP3 and MDM4 by Real-time PCR. This is the first report on the effect of miR-214 on global mRNA profile in cervical cancer cells. This study also reports new biomarkers for cervical cancer prognosis.
Identifiants
pubmed: 31465786
pii: S0888-7543(19)30413-6
doi: 10.1016/j.ygeno.2019.08.020
pii:
doi:
Substances chimiques
Biomarkers, Tumor
0
MIRN124 microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1490-1499Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.