Characterization of the endogenous retrovirus insertion in
Aromatase
Chicken
ERV
Endogenous retrovirus
Henny feather
LTR
Journal
Mobile DNA
ISSN: 1759-8753
Titre abrégé: Mob DNA
Pays: England
ID NLM: 101519891
Informations de publication
Date de publication:
2019
2019
Historique:
received:
20
05
2019
accepted:
14
08
2019
entrez:
31
8
2019
pubmed:
31
8
2019
medline:
31
8
2019
Statut:
epublish
Résumé
Henny feathering in chickens is determined by a dominant mutation that transforms male-specific plumage to female-like plumage. Previous studies indicated that this phenotype is caused by ectopic expression in skin of We used publicly available whole genome sequence data to determine the flanking sequences of the ERV, and then PCR amplified the entire insertion and sequenced it using Nanopore long reads and Sanger sequencing. The 7524 bp insertion contains an intact endogenous retrovirus that was not found in chickens representing 31 different breeds not showing henny feathering or in samples of the ancestral red junglefowl. The sequence shows over 99% sequence identity to the avian leukosis virus ev-1 and ev-21 strains, suggesting a recent integration. The ERV 3'LTR, containing a powerful transcriptional enhancer and core promoter with TATA box together with binding sites for EFIII and Ig/EBP inside the We demonstrate that the henny feathering allele harbors an insertion of an intact avian leukosis virus at the 5'end of
Sections du résumé
BACKGROUND
BACKGROUND
Henny feathering in chickens is determined by a dominant mutation that transforms male-specific plumage to female-like plumage. Previous studies indicated that this phenotype is caused by ectopic expression in skin of
RESULTS
RESULTS
We used publicly available whole genome sequence data to determine the flanking sequences of the ERV, and then PCR amplified the entire insertion and sequenced it using Nanopore long reads and Sanger sequencing. The 7524 bp insertion contains an intact endogenous retrovirus that was not found in chickens representing 31 different breeds not showing henny feathering or in samples of the ancestral red junglefowl. The sequence shows over 99% sequence identity to the avian leukosis virus ev-1 and ev-21 strains, suggesting a recent integration. The ERV 3'LTR, containing a powerful transcriptional enhancer and core promoter with TATA box together with binding sites for EFIII and Ig/EBP inside the
CONCLUSIONS
CONCLUSIONS
We demonstrate that the henny feathering allele harbors an insertion of an intact avian leukosis virus at the 5'end of
Identifiants
pubmed: 31467598
doi: 10.1186/s13100-019-0181-4
pii: 181
pmc: PMC6712707
doi:
Types de publication
Journal Article
Langues
eng
Pagination
38Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interest.
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