A 2x folic acid treatment affects epigenetics and dendritic spine densities in SHSY5Y cells.
ASD, Autism Spectrum Disorders
DNA methylation
DNMT, DNA Methyltransferase
Dendritic spines
Epigenetics
FA, Folic Acid
Folic acid
Gene expression
HAT, Histone Acetyltransferase
HDAC, Histone Deacetylase
HMT, Histone Methyltransferase
Histone modifications
MBD2, Methyl-CpG Binding Domain Protein 2
MECP2, Methyl-CpG Binding Protein 2
Journal
Biochemistry and biophysics reports
ISSN: 2405-5808
Titre abrégé: Biochem Biophys Rep
Pays: Netherlands
ID NLM: 101660999
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
15
02
2019
revised:
20
05
2019
accepted:
12
08
2019
entrez:
31
8
2019
pubmed:
31
8
2019
medline:
31
8
2019
Statut:
epublish
Résumé
Many diseases are now associated with aberrant epigenetics and gene expression changes. Epigenetics can be modified by factors like diet. One dietary factor, folic acid, is consumed in various forms including supplements, energy drinks, and fortified grains. It was hypothesized high levels of folic acid would affect gene expression and enzyme activity of chromatin modifying enzymes as well as dendritic spine densities in a commonly utilized neuron model, the SHSY5Y cell. Decreased MBD2 and MECP2 were discovered upon treatment of SHSY5Y cells with a 2x folic acid dose. Corresponding decreases in dendritic spines were apparent in the 2x folic acid treated cells as well. Activity of DNMTs and H3K4 HMTs was altered. Further, H3K4me1, H3K4me3, H3K9Ac, and global DNA methylation were decreased in the 2x folic acid treated cells. Further studies are warranted to determine if the effects of excess folic acid are detrimental to organismal physiology.
Identifiants
pubmed: 31467993
doi: 10.1016/j.bbrep.2019.100681
pii: S2405-5808(19)30040-8
pii: 100681
pmc: PMC6711848
doi:
Types de publication
Journal Article
Langues
eng
Pagination
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