Structural Insights into Curli CsgA Cross-β Fibril Architecture Inspire Repurposing of Anti-amyloid Compounds as Anti-biofilm Agents.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
08
03
2019
accepted:
11
07
2019
revised:
17
09
2019
pubmed:
31
8
2019
medline:
16
1
2020
entrez:
31
8
2019
Statut:
epublish
Résumé
Curli amyloid fibrils secreted by Enterobacteriaceae mediate host cell adhesion and contribute to biofilm formation, thereby promoting bacterial resistance to environmental stressors. Here, we present crystal structures of amyloid-forming segments from the major curli subunit, CsgA, revealing steric zipper fibrils of tightly mated β-sheets, demonstrating a structural link between curli and human pathological amyloids. D-enantiomeric peptides, originally developed to interfere with Alzheimer's disease-associated amyloid-β, inhibited CsgA fibrillation and reduced biofilm formation in Salmonella typhimurium. Moreover, as previously shown, CsgA fibrils cross-seeded fibrillation of amyloid-β, providing support for the proposed structural resemblance and potential for cross-species amyloid interactions. The presented findings provide structural insights into amyloidogenic regions important for curli formation, suggest a novel strategy for disrupting amyloid-structured biofilms, and hypothesize on the formation of self-propagating prion-like species originating from a microbial source that could influence neurodegenerative diseases.
Identifiants
pubmed: 31469892
doi: 10.1371/journal.ppat.1007978
pii: PPATHOGENS-D-19-00475
pmc: PMC6748439
doi:
Substances chimiques
Amyloid
0
Escherichia coli Proteins
0
Peptide Fragments
0
csgA protein, E coli
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1007978Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Sci Rep. 2015 Sep 23;5:13222
pubmed: 26394756
ACS Chem Neurosci. 2010 Sep 15;1(9):639-48
pubmed: 22778851
Sci Rep. 2017 Oct 19;7(1):13537
pubmed: 29051531
J Mol Biol. 2005 Sep 16;352(2):245-52
pubmed: 16083908
Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):E1994-2003
pubmed: 25855634
J Am Chem Soc. 2016 Aug 3;138(30):9663-74
pubmed: 27355699
Res Microbiol. 2012 Nov-Dec;163(9-10):592-606
pubmed: 23108148
J Alzheimers Dis. 2018;64(2):363-366
pubmed: 29889070
Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):E5439-44
pubmed: 25453093
Nat Struct Mol Biol. 2009 Sep;16(9):973-8
pubmed: 19684598
Science. 2017 Feb 24;355(6327):831-833
pubmed: 28232575
Biochemistry. 2011 Oct 4;50(39):8281-90
pubmed: 21877724
J Mol Biol. 2008 Jul 4;380(2):425-36
pubmed: 18514226
Angew Chem Int Ed Engl. 2015 Dec 1;54(49):14669-72
pubmed: 26474178
Cell Mol Life Sci. 2013 Aug;70(16):2999-3012
pubmed: 23334185
J Mol Biol. 1999 Jul 16;290(3):741-56
pubmed: 10395827
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
PLoS Pathog. 2012;8(6):e1002744
pubmed: 22685403
J Phys Chem Lett. 2014 Jun 5;5(11):1984-1993
pubmed: 24932380
Front Cell Infect Microbiol. 2015 Apr 08;5:33
pubmed: 25905048
J Am Chem Soc. 2010 Oct 6;132(39):13765-75
pubmed: 20828131
Nature. 2017 Jul 13;547(7662):185-190
pubmed: 28678775
Nat Struct Mol Biol. 2015 Jun;22(6):499-505
pubmed: 25938662
Chemistry. 2012 Apr 10;18(15):4522-32
pubmed: 22431310
Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):163-8
pubmed: 19966296
Cell Mol Life Sci. 2011 Apr;68(8):1429-38
pubmed: 20853129
Methods Mol Biol. 2017;1523:89-100
pubmed: 27975245
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Science. 2008 Mar 14;319(5869):1523-6
pubmed: 18339938
Annu Rev Microbiol. 2006;60:131-47
pubmed: 16704339
Mini Rev Med Chem. 2012 May;12(5):388-98
pubmed: 22303971
J Mol Biol. 2010 Jan 29;395(4):717-27
pubmed: 19781557
Science. 2009 Jul 17;325(5938):328-32
pubmed: 19541956
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
pubmed: 15299926
J Infect Dis. 2015 Feb 15;211(4):641-50
pubmed: 25214518
Aging (Albany NY). 2016 Apr;8(4):575-88
pubmed: 26961231
J Mol Biol. 2018 May 11;430(10):1431-1441
pubmed: 29625200
J Am Chem Soc. 2015 Jan 14;137(1):22-5
pubmed: 25415595
Med Microbiol Immunol. 1998 Jun;187(1):23-42
pubmed: 9749980
Biophys J. 2010 Aug 9;99(4):1239-46
pubmed: 20713008
Mol Neurobiol. 2019 Mar;56(3):1841-1851
pubmed: 29936690
Sci Transl Med. 2016 May 25;8(340):340ra72
pubmed: 27225182
J Oral Microbiol. 2015 Sep 17;7:29143
pubmed: 26385886
Nature. 2007 May 24;447(7143):453-7
pubmed: 17468747
J Biol Chem. 2007 Feb 9;282(6):3713-9
pubmed: 17164238
J Alzheimers Dis. 2014;39(1):169-79
pubmed: 24150108
Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):3237-3242
pubmed: 29531041
J Alzheimers Dis. 2015;48(2):319-53
pubmed: 26401998
Nature. 2013 Sep 5;501(7465):45-51
pubmed: 24005412
Nat Chem Biol. 2009 Dec;5(12):913-9
pubmed: 19915538
Cell. 2016 Nov 3;167(4):915-932
pubmed: 27814521
Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11858-63
pubmed: 26351699
Anal Bioanal Chem. 2016 Nov;408(27):7709-7717
pubmed: 27580606
J Mol Biol. 2010 Sep 17;402(2):311-25
pubmed: 20600104
Alzheimers Dement. 2015 Jun;11(6):587-92
pubmed: 25304990
J Alzheimers Dis. 2013;34(3):609-20
pubmed: 23271316
Curr Opin Microbiol. 2013 Feb;16(1):85-92
pubmed: 23434114
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13363-83
pubmed: 9811807
J Alzheimers Dis Parkinsonism. 2015 Mar;5(1):177
pubmed: 25977840
ACS Chem Neurosci. 2017 Sep 20;8(9):1889-1900
pubmed: 28581708
Trends Biochem Sci. 1999 Sep;24(9):329-32
pubmed: 10470028
PLoS One. 2015 Oct 20;10(10):e0140388
pubmed: 26485271
Nutr Rev. 2016 Oct;74(10):624-34
pubmed: 27634977
J Biol Chem. 2012 Oct 12;287(42):35092-103
pubmed: 22891247
Science. 2017 Oct 6;358(6359):116-119
pubmed: 28882996
Front Aging Neurosci. 2017 Oct 24;9:336
pubmed: 29114218
J Bacteriol. 1991 Aug;173(15):4773-81
pubmed: 1677357
Protein Sci. 2004 Dec;13(12):3314-21
pubmed: 15537750
Appl Environ Microbiol. 1997 Apr;63(4):1570-6
pubmed: 16535579
Structure. 2011 Sep 7;19(9):1307-16
pubmed: 21893289
Curr Opin Struct Biol. 2006 Feb;16(1):118-26
pubmed: 16434184
Adv Protein Chem Struct Biol. 2012;88:133-52
pubmed: 22814708
Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7130-5
pubmed: 27298344
Curr Opin Struct Biol. 2006 Apr;16(2):260-5
pubmed: 16563741
Protein Sci. 2013 Jun;22(6):851-8
pubmed: 23526461
Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12494-9
pubmed: 17636121
Chem Biol. 2013 Jan 24;20(1):102-10
pubmed: 23352144
Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E4976-84
pubmed: 27469165
Adv Protein Chem. 1981;34:167-339
pubmed: 7020376
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3487-92
pubmed: 20133726
Mol Microbiol. 2001 Mar;39(6):1452-63
pubmed: 11260463
J Am Chem Soc. 2012 Feb 22;134(7):3439-44
pubmed: 22260746
Science. 2002 Feb 1;295(5556):851-5
pubmed: 11823641
Biochem J. 2009 Jul 15;421(3):415-23
pubmed: 19435461
Chem Biol. 2013 Oct 24;20(10):1245-54
pubmed: 24035282
Sci Rep. 2016 Apr 21;6:24656
pubmed: 27098162
Biochim Biophys Acta. 2016 Nov;1858(11):2717-2724
pubmed: 27423267
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
PLoS One. 2016 Mar 10;11(3):e0151081
pubmed: 26963387
J Mol Biol. 1993 Dec 20;234(4):946-50
pubmed: 8263940
Nat Chem Biol. 2018 Sep;14(9):833-834
pubmed: 30120359
J Nat Sci. 2015 Jul;1(7):
pubmed: 26097896
Annu Rev Biochem. 2017 Jun 20;86:69-95
pubmed: 28125289
PLoS Pathog. 2017 Dec 21;13(12):e1006654
pubmed: 29267402
Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3329-34
pubmed: 22328156
J Mol Biol. 2008 Jul 11;380(3):570-80
pubmed: 18565345
ChemMedChem. 2008 Dec;3(12):1848-52
pubmed: 19016284
Curr Opin Microbiol. 2013 Feb;16(1):93-9
pubmed: 23313395
PLoS One. 2010 Oct 26;5(10):e13625
pubmed: 21049018
Front Neurol. 2014 Apr 04;5:43
pubmed: 24772103
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6562-6
pubmed: 8692856
Biochim Biophys Acta. 2013 Oct;1828(10):2328-38
pubmed: 23746423
Mol Cell. 2015 Feb 5;57(3):445-55
pubmed: 25620560
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
J Mol Biol. 2018 Oct 12;430(20):3631-3641
pubmed: 30017921
Am J Clin Pathol. 1965 Jul;44:59-61
pubmed: 14314221
Biochem J. 2012 May 1;443(3):719-26
pubmed: 22316405
FEBS Lett. 2018 Mar;592(6):1020-1029
pubmed: 29427517
J Alzheimers Dis. 2015;45(2):349-62
pubmed: 25589730
Eur J Pharm Sci. 2017 Sep 30;107:203-207
pubmed: 28711713
Nat Commun. 2018 Aug 29;9(1):3512
pubmed: 30158633
Nat Commun. 2018 Feb 16;9(1):699
pubmed: 29453354
Sci Rep. 2015 Nov 23;5:16569
pubmed: 26593271
PeerJ. 2013 Feb 12;1:e5
pubmed: 23638387
J Dairy Res. 2013 Nov;80(4):496-502
pubmed: 24103631
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15463-8
pubmed: 14665689
Nat Methods. 2010 Mar;7(3):237-42
pubmed: 20154676
Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18349-54
pubmed: 19015532
Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):6098-102
pubmed: 15829582
Cell. 2012 Mar 16;148(6):1188-203
pubmed: 22424229
Protein Eng Des Sel. 2008 Apr;21(4):241-6
pubmed: 18252750
Infect Immun. 2013 Feb;81(2):496-504
pubmed: 23208606
Front Aging Neurosci. 2015 Feb 10;7:9
pubmed: 25713531
Nat Commun. 2018 Jan 16;9(1):234
pubmed: 29339755
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Front Microbiol. 2014 Aug 01;5:401
pubmed: 25136340
Mol Cell. 2015 Mar 19;57(6):1011-1021
pubmed: 25728769
PLoS One. 2016 Apr 22;11(4):e0153035
pubmed: 27105346
Science. 2007 Dec 21;318(5858):1900-3
pubmed: 18096801
Nat Biotechnol. 2004 Oct;22(10):1302-6
pubmed: 15361882
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17897-902
pubmed: 17098869
Sci Rep. 2016 Oct 06;6:34477
pubmed: 27708338
J Biol Chem. 2009 Sep 11;284(37):25065-76
pubmed: 19574225
J Alzheimers Dis. 2015;43(3):725-38
pubmed: 25125469
Pharm Res. 2016 Feb;33(2):328-36
pubmed: 26381279
Front Neurosci. 2017 Mar 23;11:151
pubmed: 28386215
Amyloid. 2008 Jun;15(2):77-83
pubmed: 18484333
Nature. 2014 Dec 11;516(7530):250-3
pubmed: 25219853
Mol Microbiol. 1998 Apr;28(2):249-64
pubmed: 9622351
Trends Microbiol. 2012 Feb;20(2):66-73
pubmed: 22197327
Sci Rep. 2017 May 30;7(1):2493
pubmed: 28559586