Leuprolide Acetate, a GnRH Agonist, Improves the Neurogenic Bowel in Ovariectomized Rats with Spinal Cord Injury.
Cecum
Electromyography
External anal sphincter
GnRH
Neuroregeneration
SCI
Journal
Digestive diseases and sciences
ISSN: 1573-2568
Titre abrégé: Dig Dis Sci
Pays: United States
ID NLM: 7902782
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
06
11
2018
accepted:
07
08
2019
pubmed:
1
9
2019
medline:
23
7
2020
entrez:
1
9
2019
Statut:
ppublish
Résumé
Electromyographic studies have shown that external anal sphincter activity is modified in response to distension in animals with spinal cord injury. Gonadotropin-releasing hormone and its agonist leuprolide acetate have neurotrophic properties in animals with spinal cord injury. This study was to determine the effects of leuprolide acetate treatment on electromyographic activity of the external anal sphincter and anorectal manometry in ovariectomized rats with spinal cord injury. Adult ovariectomized rats were divided in three groups: (a) sham of spinal cord injury, (b) spinal cord injury treated with saline solution, and (c) spinal cord injury treated with leuprolide acetate. The spinal cord injury was induced by clamping at level T9. Leuprolide acetate dosage of 10 μg/kg was proctored intramuscular for 5 weeks, commencing the day after the lesion. Electromyography of the external anal sphincter, anorectal manometry, and volume of the cecum were evaluated in all groups. The electromyographic study of the external anal sphincter activity showed a significant improvement in injured rats treated with leuprolide acetate. Manometric analysis and cecum volume data obtained in animals with leuprolide acetate were very similar to those found in the sham group. These results demonstrate that leuprolide acetate treatment improves the neurogenic colon in ovariectomized rats with spinal cord injury.
Sections du résumé
BACKGROUND
Electromyographic studies have shown that external anal sphincter activity is modified in response to distension in animals with spinal cord injury. Gonadotropin-releasing hormone and its agonist leuprolide acetate have neurotrophic properties in animals with spinal cord injury.
AIM
This study was to determine the effects of leuprolide acetate treatment on electromyographic activity of the external anal sphincter and anorectal manometry in ovariectomized rats with spinal cord injury.
METHODS
Adult ovariectomized rats were divided in three groups: (a) sham of spinal cord injury, (b) spinal cord injury treated with saline solution, and (c) spinal cord injury treated with leuprolide acetate. The spinal cord injury was induced by clamping at level T9. Leuprolide acetate dosage of 10 μg/kg was proctored intramuscular for 5 weeks, commencing the day after the lesion. Electromyography of the external anal sphincter, anorectal manometry, and volume of the cecum were evaluated in all groups.
RESULTS
The electromyographic study of the external anal sphincter activity showed a significant improvement in injured rats treated with leuprolide acetate. Manometric analysis and cecum volume data obtained in animals with leuprolide acetate were very similar to those found in the sham group.
CONCLUSIONS
These results demonstrate that leuprolide acetate treatment improves the neurogenic colon in ovariectomized rats with spinal cord injury.
Identifiants
pubmed: 31471861
doi: 10.1007/s10620-019-05783-4
pii: 10.1007/s10620-019-05783-4
doi:
Substances chimiques
Gonadotropin-Releasing Hormone
33515-09-2
Leuprolide
EFY6W0M8TG
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
423-430Références
Dig Dis Sci. 1998 Jun;43(6):1347-55
pubmed: 9635630
Dig Dis Sci. 1989 May;34(5):761-6
pubmed: 2496961
Spinal Cord. 2010 Oct;48(10):718-33
pubmed: 20212501
Neurosci Lett. 2009 Sep 11;461(1):21-4
pubmed: 19539704
Front Endocrinol (Lausanne). 2017 Jun 07;8:110
pubmed: 28638366
J Neurotrauma. 1995 Feb;12(1):1-21
pubmed: 7783230
J Pharm Sci. 1996 Oct;85(10):1044-8
pubmed: 8897268
Clin Pharmacokinet. 2002;41(7):485-504
pubmed: 12083977
Neurosci Lett. 2012 May 2;515(2):187-90
pubmed: 22480691
Neurochem Res. 2008 Jun;33(6):1051-6
pubmed: 18157692
Dig Dis Sci. 1994 Jun;39(6):1163-70
pubmed: 8200248
Neurourol Urodyn. 2018 Jun;37(5):1574-1582
pubmed: 30133853
Spine (Phila Pa 1976). 2007 Dec 1;32(25):2853-9
pubmed: 18246008
Neurochem Res. 2016 Oct;41(10):2693-2698
pubmed: 27339868
Neurosci Lett. 2007 Jan 3;411(1):22-5
pubmed: 17110036
Life Sci. 2001 Mar 2;68(15):1727-34
pubmed: 11270619
Spinal Cord. 2000 Oct;38(10):573-80
pubmed: 11093318
Am J Gastroenterol. 2006 Oct;101(10):2290-9
pubmed: 17032195
Gastroenterol Hepatol. 2012 May;35(5):330-6
pubmed: 22296768
Exp Neurol. 2005 May;193(1):29-42
pubmed: 15817262
Neural Regen Res. 2015 Nov;10(11):1819-24
pubmed: 26807118
CNS Neurol Disord Drug Targets. 2010 Nov;9(5):651-60
pubmed: 20632963
Dis Colon Rectum. 2009 Jul;52(7):1321-9
pubmed: 19571711
J Neurotrauma. 1998 Jun;15(6):451-7
pubmed: 9624630
Curr Gastroenterol Rep. 2018 Aug 29;20(10):47
pubmed: 30159690