Improvement of cell counting method for Neubauer counting chamber.
Neubauer counting chamber
border cells
cell counting method
distributing uniformity
errors
Journal
Journal of clinical laboratory analysis
ISSN: 1098-2825
Titre abrégé: J Clin Lab Anal
Pays: United States
ID NLM: 8801384
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
02
07
2019
revised:
07
08
2019
accepted:
08
08
2019
pubmed:
1
9
2019
medline:
7
10
2020
entrez:
1
9
2019
Statut:
ppublish
Résumé
We compared the cell counting accuracy of the conventional method and the improved method by using Neubauer counting chamber. In the improved method, all the border cells were counted and then divided by two; while, in the conventional method, only border cells on the two boundaries (top and left) were counted. About 55.814% of the samples showed more accurate results by improved counting method, about 38.372% had more accurate results by conventional counting method, and about 5.814% were counted with similar counting error by both methods. The improved method had significantly smaller counting error than conventional method (P < .05). The distribution ratio of the border cells was an independent factor for counting accuracy (P < .05). Together, the improved counting method can reduce the counting error of the Neubauer counting chamber to some extent, assess the distributing uniformity of border cells, and help to eliminate the samples with large differences in distribution.
Sections du résumé
BACKGROUND
BACKGROUND
We compared the cell counting accuracy of the conventional method and the improved method by using Neubauer counting chamber.
METHODS
METHODS
In the improved method, all the border cells were counted and then divided by two; while, in the conventional method, only border cells on the two boundaries (top and left) were counted.
RESULTS
RESULTS
About 55.814% of the samples showed more accurate results by improved counting method, about 38.372% had more accurate results by conventional counting method, and about 5.814% were counted with similar counting error by both methods. The improved method had significantly smaller counting error than conventional method (P < .05). The distribution ratio of the border cells was an independent factor for counting accuracy (P < .05).
CONCLUSION
CONCLUSIONS
Together, the improved counting method can reduce the counting error of the Neubauer counting chamber to some extent, assess the distributing uniformity of border cells, and help to eliminate the samples with large differences in distribution.
Identifiants
pubmed: 31471934
doi: 10.1002/jcla.23024
pmc: PMC6977140
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23024Subventions
Organisme : 2017 Key Project of the Chongqing Health Economics Association
ID : YWJK2017-1
Organisme : Chongqing Medical University Student Innovation Experiment Project
ID : 201664
Informations de copyright
© 2019 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc.
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