Thermal stability of cytokines: A review.
C-Reactive Protein
/ metabolism
Chemokines
/ blood
Cytokines
/ blood
Epidermal Growth Factor
/ blood
Freezing
/ adverse effects
Humans
Interferons
/ blood
Interleukin-1
/ blood
Interleukin-6
/ blood
Protein Stability
Specimen Handling
/ methods
Temperature
Transforming Growth Factor beta1
/ blood
Tumor Necrosis Factor-alpha
/ blood
Cytokines
Stability
Storage
Journal
Cytokine
ISSN: 1096-0023
Titre abrégé: Cytokine
Pays: England
ID NLM: 9005353
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
27
03
2019
revised:
22
08
2019
accepted:
23
08
2019
pubmed:
1
9
2019
medline:
21
5
2021
entrez:
1
9
2019
Statut:
ppublish
Résumé
The role of cytokines in various disease states is a burgeoning field of academic study and clinical application, however there are no consensus documents on how certain cytokines should be stored prior to quantification. This information is especially of interest to researchers assembling a biobank or clinicians who have to transport specimens to a different location in order to be tested. To review the literature and synthesize prior findings on cytokine storage and freeze/thaw stability. We searched PubMed for articles related to cytokine storage stability. All articles were analyzed for cytokines studied, source of reported cytokine concentration (i.e., human whole blood or serum, concentrations from other species or bodily sources were excluded), and reported statistical results. We identified and synthesized results of 23 peer-reviewed articles which published data on the storage and freeze/thaw stability of 33 different cytokines and chemokines. There is a wide variety of reported cytokine storage and freeze/thaw stability. Interleukin-6 and tumor necrosis factor alpha are the most widely studied cytokines in regard to temperature stability. In a few cytokines, a clear consensus can be reached as to storage safety at particular temperatures, but in most, more research needs to be done and we advise the clinician or researcher to use caution in interpreting cytokine concentration results after a long period of storage or several freeze/thaw cycles.
Sections du résumé
BACKGROUND
The role of cytokines in various disease states is a burgeoning field of academic study and clinical application, however there are no consensus documents on how certain cytokines should be stored prior to quantification. This information is especially of interest to researchers assembling a biobank or clinicians who have to transport specimens to a different location in order to be tested.
OBJECTIVE
To review the literature and synthesize prior findings on cytokine storage and freeze/thaw stability.
DESIGN
We searched PubMed for articles related to cytokine storage stability. All articles were analyzed for cytokines studied, source of reported cytokine concentration (i.e., human whole blood or serum, concentrations from other species or bodily sources were excluded), and reported statistical results.
RESULTS
We identified and synthesized results of 23 peer-reviewed articles which published data on the storage and freeze/thaw stability of 33 different cytokines and chemokines.
CONCLUSION
There is a wide variety of reported cytokine storage and freeze/thaw stability. Interleukin-6 and tumor necrosis factor alpha are the most widely studied cytokines in regard to temperature stability. In a few cytokines, a clear consensus can be reached as to storage safety at particular temperatures, but in most, more research needs to be done and we advise the clinician or researcher to use caution in interpreting cytokine concentration results after a long period of storage or several freeze/thaw cycles.
Identifiants
pubmed: 31472404
pii: S1043-4666(19)30258-3
doi: 10.1016/j.cyto.2019.154829
pii:
doi:
Substances chimiques
Chemokines
0
Cytokines
0
Interleukin-1
0
Interleukin-6
0
Transforming Growth Factor beta1
0
Tumor Necrosis Factor-alpha
0
Epidermal Growth Factor
62229-50-9
C-Reactive Protein
9007-41-4
Interferons
9008-11-1
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
154829Informations de copyright
Published by Elsevier Ltd.