Stability of placental growth factor, soluble fms-like tyrosine kinase 1, and soluble fms-like tyrosine kinase 1 e15a in human serum and plasma.
Anti-angiogenic factors
PlGF
Preeclampsia
sFlt-1
sFlt-1 e15a
Journal
Placenta
ISSN: 1532-3102
Titre abrégé: Placenta
Pays: Netherlands
ID NLM: 8006349
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
09
06
2019
revised:
30
07
2019
accepted:
01
08
2019
pubmed:
3
9
2019
medline:
25
8
2020
entrez:
3
9
2019
Statut:
ppublish
Résumé
Placental growth factor (PlGF), total soluble fms-like tyrosine-kinase 1 (sFlt-1) and its placental-specific variant, sFlt-1 e15a, show promise as biomarkers for the prediction and diagnosis of preeclampsia. This study describes the degradation of PlGF, sFlt-1 and sFlt-1 e15a within maternal serum and plasma to assist clinical implementation. Whole blood was refrigerated at 4 °C for up to 48 h prior to centrifugation for isolation of plasma and serum. PlGF and sFlt-1 were quantified using the B.R.A.H.M.S Kryptor Compact PLUS; sFlt-1 e15a via a custom ELISA. All three analytes are stable for at least 48 h at 4 °C. Serum and plasma performed comparably.
Identifiants
pubmed: 31476387
pii: S0143-4004(19)30522-3
doi: 10.1016/j.placenta.2019.08.001
pii:
doi:
Substances chimiques
Placenta Growth Factor
144589-93-5
FLT1 protein, human
EC 2.7.10.1
Vascular Endothelial Growth Factor Receptor-1
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-3Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.