Ankyrin B and Ankyrin B variants differentially modulate intracellular and surface Cav2.1 levels.
Ankyrin B
CACNA1A
Cav2.1
Intracellular pool
Surface localization
Synapse
Journal
Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876
Informations de publication
Date de publication:
02 09 2019
02 09 2019
Historique:
received:
21
06
2019
accepted:
21
08
2019
entrez:
4
9
2019
pubmed:
4
9
2019
medline:
2
6
2020
Statut:
epublish
Résumé
Ankyrin B (AnkB) is an adaptor and scaffold for motor proteins and various ion channels that is ubiquitously expressed, including in the brain. AnkB has been associated with neurological disorders such as epilepsy and autism spectrum disorder, but understanding of the underlying mechanisms is limited. Cav2.1, the pore-forming subunit of P/Q type voltage gated calcium channels, is a known interactor of AnkB and plays a crucial role in neuronal function. Here we report that wildtype AnkB increased overall Cav2.1 levels without impacting surface Cav2.1 levels in HEK293T cells. An AnkB variant, p.S646F, which we recently discovered to be associated with seizures, further increased overall Cav2.1 levels, again with no impact on surface Cav2.1 levels. AnkB p.Q879R, on the other hand, increased surface Cav2.1 levels in the presence of accessory subunits α
Identifiants
pubmed: 31477143
doi: 10.1186/s13041-019-0494-8
pii: 10.1186/s13041-019-0494-8
pmc: PMC6720858
doi:
Substances chimiques
Ankyrins
0
Calcium Channels, N-Type
0
Mutant Proteins
0
Protein Subunits
0
voltage-dependent calcium channel (P-Q type)
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
75Subventions
Organisme : CIHR
ID : MOP142215
Pays : Canada
Organisme : CIHR
ID : PJT-153392
Pays : Canada
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