The marginal cells of the Caenorhabditis elegans pharynx scavenge cholesterol and other hydrophobic small molecules.

Animals Bacteria / metabolism Caenorhabditis elegans / cytology Caenorhabditis elegans Proteins / genetics Cell Membrane / metabolism Cholesterol / metabolism Crystallization Hydrophobic and Hydrophilic Interactions Mutation Pharynx / cytology Sphingomyelins / chemistry Transferases (Other Substituted Phosphate Groups) / genetics

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
02 09 2019
Historique:
received: 02 03 2019
accepted: 09 08 2019
entrez: 4 9 2019
pubmed: 4 9 2019
medline: 31 12 2019
Statut: epublish

Résumé

The nematode Caenorhabditis elegans is a bacterivore filter feeder. Through the contraction of the worm's pharynx, a bacterial suspension is sucked into the pharynx's lumen. Excess liquid is then shunted out of the buccal cavity through ancillary channels made by surrounding marginal cells. We find that many worm-bioactive small molecules (a.k.a. wactives) accumulate inside of the marginal cells as crystals or globular spheres. Through screens for mutants that resist the lethality associated with one crystallizing wactive we identify a presumptive sphingomyelin-synthesis pathway that is necessary for crystal and sphere accumulation. We find that expression of sphingomyelin synthase 5 (SMS-5) in the marginal cells is not only sufficient for wactive accumulation but is also important for absorbing exogenous cholesterol, without which C. elegans cannot develop. We conclude that sphingomyelin-rich marginal cells act as a sink to scavenge important nutrients from filtered liquid that might otherwise be shunted back into the environment.

Identifiants

DOI: 10.1038/s41467-019-11908-0 PMID: 31477732 PMC: PMC6718421
pubmed: 31477732
doi: 10.1038/s41467-019-11908-0
pii: 10.1038/s41467-019-11908-0
pmc: PMC6718421
doi:

Substances chimiques

Caenorhabditis elegans Proteins 0
Sphingomyelins 0
Cholesterol 97C5T2UQ7J
Transferases (Other Substituted Phosphate Groups) EC 2.7.8.-
phosphatidylcholine-ceramide phosphocholine transferase EC 2.7.8.-

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3938

Subventions

Organisme : NIH HHS
ID : R24 OD010943
Pays : United States
Organisme : NCRR NIH HHS
ID : R24 RR012596
Pays : United States
Organisme : CIHR
ID : 376634
Pays : Canada
Organisme : CIHR
ID : 313296
Pays : Canada

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Auteurs

Muntasir Kamal (M)

Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Houtan Moshiri (H)

Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Lilia Magomedova (L)

Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, ON, M5S 3M2, Canada.

Duhyun Han (D)

The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, M5S 1A8, Canada.

Ken C Q Nguyen (KCQ)

Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, 10461, USA.

May Yeo (M)

Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Jessica Knox (J)

Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Rachel Bagg (R)

Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Amy M Won (AM)

The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Department of Biochemistry, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Karolina Szlapa (K)

Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Christopher M Yip (CM)

The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Department of Biochemistry, University of Toronto, Toronto, ON, M5S 3E1, Canada.

Carolyn L Cummins (CL)

Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, ON, M5S 3M2, Canada.
ORCID: 0000-0001-7603-6577

David H Hall (DH)

Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, 10461, USA.
ORCID: 0000-0001-8459-9820

Peter J Roy (PJ)

Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A8, Canada. peter.roy@utoronto.ca.
The Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, M5S 3E1, Canada. peter.roy@utoronto.ca.
Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, M5S 1A8, Canada. peter.roy@utoronto.ca.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Bactéries › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Helminthes › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Caenorhabditis › Caenorhabditis elegans › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'helminthes › Protéines de Caenorhabditis elegans › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Lipides › Lipides membranaires › Stérols › Cholestérol › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Phénomènes chimiques › Cristallisation › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Système stomatognathique › Pharynx › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Lipides › Lipides membranaires › Sphingolipides › Glycosphingolipides › Glycosphingolipides neutres › Sphingomyéline › The marginal cells of the Caenorhabditis...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Transferases (other substituted phosphate groups) › The marginal cells of the Caenorhabditis...