Prevalence of Giardia and Cryptosporidium in young livestock and dogs in Magude District of Maputo Province, Mozambique.
Cryptosporidium
Giardia
Mozambique.
calves
dogs
goats
zoonoses
Journal
The Onderstepoort journal of veterinary research
ISSN: 2219-0635
Titre abrégé: Onderstepoort J Vet Res
Pays: South Africa
ID NLM: 0401107
Informations de publication
Date de publication:
12 Aug 2019
12 Aug 2019
Historique:
received:
15
10
2018
accepted:
11
02
2019
revised:
04
02
2019
entrez:
4
9
2019
pubmed:
4
9
2019
medline:
3
3
2020
Statut:
epublish
Résumé
Giardia and Cryptosporidium species are significant zoonotic parasites of humans and domesticated animals. The study aimed to determine the prevalence of Giardia and Cryptosporidium in livestock and dogs of the Magude District. The flotation technique (Willis), modified Ziehl-Neelsen (mZN) and direct and indirect immunofluorescence (DIF and IIF) techniques were applied to determine the prevalence of Giardia and Cryptosporidium species in faecal samples of dog pups (156), goat kids (60) and calves (480) from the Magude District of Mozambique from February to September 2015. Using Willis, IIF and DIF, the prevalence of Giardia in calves was 0%, 8.1%, and 6.0%; in dogs 0.6%, 8.3% and 5.7% and for goats 0% and 13.3% (IIF was not performed), respectively. The prevalence of Cryptosporidium in calves using Willis, mZN, IIF and DIF was 0%, 3.8%, 4.7% and 0.4% and in dogs 0%, 0.6%, 6.4% and 0.6%, respectively. The parasite was not detected in goats. Results from the present study showed that IIF performed better diagnosis of Giardia and Cryptosporidium, and that the mZN can be used as an alternative for Cryptosporidium because of the high cost of IIF. There is a need for identification of genotypes or subtypes of these parasites through application of molecular techniques in order to determine their zoonotic potential, and we advocate a 'one health' approach in the control and prevention of these parasites.
Sections du résumé
BACKGROUND
BACKGROUND
Giardia and Cryptosporidium species are significant zoonotic parasites of humans and domesticated animals.
OBJECTIVES
OBJECTIVE
The study aimed to determine the prevalence of Giardia and Cryptosporidium in livestock and dogs of the Magude District.
METHOD
METHODS
The flotation technique (Willis), modified Ziehl-Neelsen (mZN) and direct and indirect immunofluorescence (DIF and IIF) techniques were applied to determine the prevalence of Giardia and Cryptosporidium species in faecal samples of dog pups (156), goat kids (60) and calves (480) from the Magude District of Mozambique from February to September 2015.
RESULTS
RESULTS
Using Willis, IIF and DIF, the prevalence of Giardia in calves was 0%, 8.1%, and 6.0%; in dogs 0.6%, 8.3% and 5.7% and for goats 0% and 13.3% (IIF was not performed), respectively. The prevalence of Cryptosporidium in calves using Willis, mZN, IIF and DIF was 0%, 3.8%, 4.7% and 0.4% and in dogs 0%, 0.6%, 6.4% and 0.6%, respectively. The parasite was not detected in goats.
CONCLUSION
CONCLUSIONS
Results from the present study showed that IIF performed better diagnosis of Giardia and Cryptosporidium, and that the mZN can be used as an alternative for Cryptosporidium because of the high cost of IIF. There is a need for identification of genotypes or subtypes of these parasites through application of molecular techniques in order to determine their zoonotic potential, and we advocate a 'one health' approach in the control and prevention of these parasites.
Identifiants
pubmed: 31478737
doi: 10.4102/ojvr.v86i1.1709
pmc: PMC6739555
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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