A High-Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity.
drug discovery
high-throughput screening
stem cell biotechnology
tissue engineering
toxicology
Journal
Current protocols in stem cell biology
ISSN: 1938-8969
Titre abrégé: Curr Protoc Stem Cell Biol
Pays: United States
ID NLM: 101470226
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
entrez:
4
9
2019
pubmed:
4
9
2019
medline:
10
9
2020
Statut:
ppublish
Résumé
This article describes a screening platform to identify compounds that affect human embryonic vascular development. The procedure comprises the generation of human embryonic-like endothelial cells (ECs) from human pluripotent stem cells (hPSCs) and subsequent maturation under arterial flow conditions; the use of these cells for the high-throughput screening of small molecules that specifically inhibit the survival of embryonic-like ECs; the confirmation of the hits in embryonic-like ECs cultured under flow shear stress; and final validation in mouse embryonic ECs. The embryonic-like ECs express embryonic genes including DLL1, EPHB2, LYN, TEK, ID1, NRP2, CAST, FLT1, IGF1, DKK3, NIN, LEF1, and SORBS3. The entire screening procedure (without the validation step) can be completed within 1 month. This platform is an alternative/complement to standard animal protocols for assessing the effects of chemicals on embryonic vascular development. © 2019 by John Wiley & Sons, Inc.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e93Informations de copyright
© 2019 John Wiley & Sons, Inc.
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