Glomerular Collagen Deposition and Lipocalin-2 Expression Are Early Signs of Renal Injury in Prediabetic Obese Rats.
Adipose Tissue
/ metabolism
Animals
Biomarkers
/ metabolism
Body Weight
Collagen
/ metabolism
Diet, High-Fat
Fibrosis
Gene Expression Regulation
Inflammation
/ genetics
Kidney Glomerulus
/ injuries
Lipids
/ blood
Lipocalin-2
/ metabolism
Liver
/ enzymology
Male
MicroRNAs
/ genetics
Obesity
/ blood
Oxidative Stress
/ genetics
Phosphorylation
Phosphoserine
/ metabolism
Prediabetic State
/ blood
Proto-Oncogene Proteins c-akt
/ metabolism
RNA, Messenger
/ genetics
Rats, Long-Evans
Streptozocin
collagen type IV
inflammation
lipocalin-2
obesity
renal injury
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
30 08 2019
30 08 2019
Historique:
received:
10
08
2019
accepted:
29
08
2019
entrez:
5
9
2019
pubmed:
5
9
2019
medline:
22
1
2020
Statut:
epublish
Résumé
Feeding rats with high-fat diet (HFD) with a single streptozotocin (STZ) injection induced obesity, slightly elevated fasting blood glucose and impaired glucose and insulin tolerance, and caused cardiac hypertrophy and mild diastolic dysfunction as published before by Koncsos et al. in 2016. Here we aimed to explore the renal consequences in the same groups of rats. Male Long-Evans rats were fed normal chow (CON; n = 9) or HFD containing 40% lard and were administered STZ at 20 mg/kg (i.p.) at week four (prediabetic rats, PRED, n = 9). At week 21 blood and urine samples were taken and kidney and liver samples were collected for histology, immunohistochemistry and for analysis of gene expression. HFD and STZ increased body weight and visceral adiposity and plasma leptin concentration. Despite hyperleptinemia, plasma C-reactive protein concentration decreased in PRED rats. Immunohistochemistry revealed elevated collagen IV protein expression in the glomeruli, and
Identifiants
pubmed: 31480394
pii: ijms20174266
doi: 10.3390/ijms20174266
pmc: PMC6747173
pii:
doi:
Substances chimiques
Biomarkers
0
Lipids
0
Lipocalin-2
0
MicroRNAs
0
RNA, Messenger
0
Phosphoserine
17885-08-4
Streptozocin
5W494URQ81
Collagen
9007-34-5
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Hungarian Scientific Research Fund
ID : SNN-114619
Pays : International
Organisme : Hungarian Scientific Research Fund
ID : ANN-110810
Pays : International
Organisme : Hungarian Scientific Research Fund
ID : K116525
Pays : International
Organisme : Hungarian Scientific Research Fund
ID : 109737
Pays : International
Organisme : National Research, Development and Innovation Fund of Hungary
ID : NVKP_16-1-2016-0042
Pays : International
Organisme : National Research, Development and Innovation Fund of Hungary
ID : NVKP-16-12016-0017
Pays : International
Organisme : Economic Development and Innovation Operative Program Grant
ID : GINOP 2.3.2-15-2016-00048
Pays : International
Organisme : Kispál Gyula startup grant of the University of Pécs
ID : 300021
Pays : International
Organisme : Semmelweis University
ID : EFOP-3.6.3-VEKOP-16-201700009
Pays : International
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