MicroRNA Signatures in Malignant Pleural Mesothelioma Effusions.


Journal

Disease markers
ISSN: 1875-8630
Titre abrégé: Dis Markers
Pays: United States
ID NLM: 8604127

Informations de publication

Date de publication:
2019
Historique:
received: 20 11 2018
revised: 28 05 2019
accepted: 17 07 2019
entrez: 5 9 2019
pubmed: 5 9 2019
medline: 11 2 2020
Statut: epublish

Résumé

Malignant pleural mesothelioma (MPM) is an incurable cancer of the pleura that can be difficult to diagnose. Biomarkers for an easier and/or earlier diagnosis are needed. Approximately 90% of MPM patients develop a pleural effusion (PE). PEs are ideal sources of biomarkers as the fluid would almost always require drainage for diagnostic and/or therapeutic reasons. However, differentiating MPM PE from PE caused by other diseases can be challenging. MicroRNAs are popular biomarkers given their stable expression in tissue and fluid. MicroRNAs have been analysed in PE cytology samples for the diagnosis of MPM but have not been measured in frozen/fresh PE. We hypothesise that microRNAs expressed in PE are biomarkers for MPM. TaqMan OpenArray was used to analyse over 700 microRNAs in PE cells and supernatants from 26 MPMs and 21 other PE-causing diseases. In PE cells, combining miR-143, miR-210, and miR-200c could differentiate MPM with an area under the curve (AUC) of 0.92. The three-microRNA signature could also discriminate MPM from a further 40 adenocarcinomas with an AUC of 0.9887. These results suggest that the expression of miR-143, miR-210, and miR-200c in PE cells might provide a signature for diagnosing MPM.

Identifiants

pubmed: 31481984
doi: 10.1155/2019/8628612
pmc: PMC6701424
doi:

Substances chimiques

Biomarkers, Tumor 0
MicroRNAs 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

8628612

Déclaration de conflit d'intérêts

No potential conflicts of interest were disclosed.

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Auteurs

Kimberly A Birnie (KA)

Institute for Respiratory Health and Centre for Respiratory Health, School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.

Cecilia M Prêle (CM)

Institute for Respiratory Health and Centre for Respiratory Health, School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.
Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia and Harry Perkins Institute of Medical Research, Nedlands, WA 6009, Australia.

Arthur W Bill Musk (AWB)

Occupational Respiratory Epidemiology, School of Population and Global Health, University of Western Australia, Crawley, WA 6009, Australia.
Department of Respiratory Medicine, Sir Charles Gairdner Hospital, Nedlands, WA 6009, Australia.

Nicholas de Klerk (N)

Occupational Respiratory Epidemiology, School of Population and Global Health, University of Western Australia, Crawley, WA 6009, Australia.
Telethon Kids Institute and Centre for Child Health Research, University of Western Australia, Nedlands, WA 6009, Australia.

Y C Gary Lee (YCG)

Institute for Respiratory Health and Centre for Respiratory Health, School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.
Department of Respiratory Medicine, Sir Charles Gairdner Hospital, Nedlands, WA 6009, Australia.

Deirdre Fitzgerald (D)

Department of Respiratory Medicine, Sir Charles Gairdner Hospital, Nedlands, WA 6009, Australia.

Richard J N Allcock (RJN)

School of Biomedical Sciences, University of Western Australia, and Pathwest Laboratory Medicine, QEII Medical Centre, Nedlands, WA 6009, Australia.
Pathwest Laboratory Medicine WA, QEII Medical Centre, Monash Avenue, Nedlands, WA 6009, Australia.

Philip J Thompson (PJ)

Institute for Respiratory Health and Centre for Respiratory Health, School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.

Jenette Creaney (J)

Department of Respiratory Medicine, Sir Charles Gairdner Hospital, Nedlands, WA 6009, Australia.
Medical School, QEII Medical Centre, University of Western Australia, Nedlands, WA 6009, Australia.

Bahareh Badrian (B)

Institute for Respiratory Health and Centre for Respiratory Health, School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.

Steven E Mutsaers (SE)

Institute for Respiratory Health and Centre for Respiratory Health, School of Biomedical Sciences, University of Western Australia, Nedlands, WA 6009, Australia.
Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia and Harry Perkins Institute of Medical Research, Nedlands, WA 6009, Australia.

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