The E3 ligase MuRF2 plays a key role in the functional capacity of skeletal muscle fibroblasts.
Journal
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
ISSN: 1414-431X
Titre abrégé: Braz J Med Biol Res
Pays: Brazil
ID NLM: 8112917
Informations de publication
Date de publication:
2019
2019
Historique:
received:
21
02
2019
accepted:
11
07
2019
entrez:
5
9
2019
pubmed:
5
9
2019
medline:
19
9
2019
Statut:
ppublish
Résumé
Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might function as a cytoskeleton adaptor. Here, we hypothesized that MuRF2 also plays a functional role in skeletal muscle fibroblasts. We found that skeletal muscle fibroblasts express MuRF2 and its siRNA knock-down promoted decreased fibroblast migration, cell border accumulation of polymerized actin, and down-regulation of the phospho-Akt expression. Our results indicated that MuRF2 was necessary to maintain the actin cytoskeleton functionality in skeletal muscle fibroblasts via Akt activity and exerted an important role in extracellular matrix remodeling in the skeletal muscle tissue.
Identifiants
pubmed: 31482977
pii: S0100-879X2019000900609
doi: 10.1590/1414-431X20198551
pmc: PMC6720025
pii:
doi:
Substances chimiques
Muscle Proteins
0
muscle RING finger 2 protein, mouse
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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