Recent advances in heme biocatalysis engineering.
cytochrome P450 monooxygenases (P450s)
genomic data mining
heme enzymes
heme peroxidases
peroxygenases
protein engineering
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
28
03
2019
revised:
17
07
2019
accepted:
29
08
2019
pubmed:
5
9
2019
medline:
17
9
2020
entrez:
5
9
2019
Statut:
ppublish
Résumé
Heme enzymes have the potential to be widely used as biocatalysts due to their capability to perform a vast variety of oxidation reactions. In spite of their versatility, the application of heme enzymes was long time-limited for the industry due to their low activity and stability in large scale processes. The identification of novel natural biocatalysts and recent advances in protein engineering have led to new reactions with a high application potential. The latest creation of a serine-ligated mutant of BM3 showed an efficient transfer of reactive carbenes into C═C bonds of olefins reaching total turnover numbers of more than 60,000 and product titers of up to 27 g/L
Substances chimiques
Heme
42VZT0U6YR
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
3469-3475Informations de copyright
© 2019 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.