A living plant cell-based biosensor for real-time monitoring invisible damage of plant cells under heavy metal stress.

Biosensing Techniques Metals, Heavy / analysis Plant Cells / physiology Soil Pollutants / toxicity Toxicity Tests
Heavy metals Invisible damage Living plant cell-based biosensor Plants Vitronectin-like protein

Journal

The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500

Informations de publication

Date de publication:
20 Dec 2019
Historique:
received: 12 06 2019
revised: 23 08 2019
accepted: 23 08 2019
pubmed: 5 9 2019
medline: 10 1 2020
entrez: 5 9 2019
Statut: ppublish

Résumé

Heavy metals inevitably cause invisible or visible damage to plants, leading to significant economic losses. Therefore, it is necessary to develop a method for timely monitoring the damage of plants under the stress of heavy metals. Here, vitronectin-like proteins (VN) on the surface of plant cells is as an important biomarker for monitoring damage of plants under the stress of heavy metals. A living plant cell-based biosensor is constructed to monitor invisible damage of plant cells induced by cadmium [Cd(II)] or lead [Pb(II)]. To fabricate this sensor, l-cysteine was first modified on the glassy carbon electrode followed by the modification of anti-IgG-Au antibody. Then, the living plant cells, incubated with the anti-VN, were modified onto the electrode. The sensor worked by determining the change in electrochemical impedance. Cd(II) and Pb(II) was detected in the linear dynamic range of 45-210 and 120-360 μmol·L

Identifiants

DOI: 10.1016/j.scitotenv.2019.134097 PMID: 31484090
pubmed: 31484090
pii: S0048-9697(19)34074-4
doi: 10.1016/j.scitotenv.2019.134097
pii:
doi:

Substances chimiques

Metals, Heavy 0
Soil Pollutants 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

134097

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Xiang Wang (X)

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Mengzhu Cheng (M)

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Qing Yang (Q)

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Haiyan Wei (H)

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Ao Xia (A)

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Lihong Wang (L)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Yue Ben (Y)

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.

Qing Zhou (Q)

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Zhenbiao Yang (Z)

Center for Plant Cell Biology, Institute of Integrative Genome Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.

Xiaohua Huang (X)

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address: huangxiaohuanjnu@yahoo.com.

Articles similaires

Beneath the surface: assessing pollution levels near major solid waste dumpsites in Lagos, Nigeria.

Udeme Olaniyan, May A Massoud, Ibrahim Alameddine et al.
1.00
Nigeria Environmental Monitoring Solid Waste Waste Disposal Facilities Refuse Disposal

Assessment of contamination levels, potential ecological and human health risks due to trace elements pollution in the vicinity of the Lolodorf uranium deposit, Southern Cameroon.

Bonaventure Mvogo Aloa, Jean Félix Beyala Ateba, Dieu Souffit Gondji et al.
1.00
Cameroon Humans Uranium Trace Elements Environmental Monitoring

Contamination and health risk assessment of potentially toxic elements in rice (Oryza sativa) and soil from Ashanti Region.

Portia Asare, Kofi Sarpong, Opoku Gyamfi et al.
1.00
Oryza Soil Pollutants Risk Assessment Metals, Heavy Humans

Prenatal metal exposures and kidney function in adolescence in Project Viva.

Natalie F Price, Pi-I D Lin, Andres Cardenas et al.
1.00
Humans Adolescent Female Pregnancy Prenatal Exposure Delayed Effects

Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › A living plant cell-based biosensor for...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › A living plant cell-based biosensor for...
questionsmedicales.fr › Cellules › Cellules végétales › A living plant cell-based biosensor for...
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Polluants environnementaux › Polluants du sol › A living plant cell-based biosensor for...
questionsmedicales.fr › Techniques d'investigation › Tests de toxicité › A living plant cell-based biosensor for...