CD4 Incorporation into HIV-1 Viral Particles Exposes Envelope Epitopes Recognized by CD4-Induced Antibodies.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
15 11 2019
Historique:
received: 20 08 2019
accepted: 29 08 2019
pubmed: 6 9 2019
medline: 21 7 2020
entrez: 6 9 2019
Statut: epublish

Résumé

CD4 downregulation on infected cells is a highly conserved function of primate lentiviruses. It has been shown to positively impact viral replication by a variety of mechanisms, including enhanced viral release and infectivity, decrease of cell reinfection, and protection from antibody-dependent cellular cytotoxicity (ADCC), which is often mediated by antibodies that require CD4 to change envelope (Env) conformation. Here, we report that incorporation of CD4 into HIV-1 viral particles affects Env conformation resulting in the exposure of occluded epitopes recognized by CD4-induced antibodies. This translates into enhanced neutralization susceptibility by these otherwise nonneutralizing antibodies but is prevented by the HIV-1 Nef accessory protein. Altogether, these findings suggest that another functional consequence of Nef-mediated CD4 downregulation is the protection of viral particles from neutralization by commonly elicited CD4-induced antibodies.

Identifiants

pubmed: 31484748
pii: JVI.01403-19
doi: 10.1128/JVI.01403-19
pmc: PMC6819941
pii:
doi:

Substances chimiques

CD4 Antigens 0
Epitopes 0
HIV Antibodies 0
HIV Envelope Protein gp120 0
Viral Envelope Proteins 0
env Gene Products, Human Immunodeficiency Virus 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : P01 AI150471
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI129769
Pays : United States
Organisme : NIAID NIH HHS
ID : R33 AI129017
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI124982
Pays : United States
Organisme : CIHR
ID : 352417
Pays : Canada
Organisme : NIAID NIH HHS
ID : R01 AI145547
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116274
Pays : United States

Informations de copyright

Copyright © 2019 American Society for Microbiology.

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Auteurs

Shilei Ding (S)

Centre de Recherche du CHUM, Montreal, Quebec, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Quebec, Canada.

Romain Gasser (R)

Centre de Recherche du CHUM, Montreal, Quebec, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Quebec, Canada.

Gabrielle Gendron-Lepage (G)

Centre de Recherche du CHUM, Montreal, Quebec, Canada.

Halima Medjahed (H)

Centre de Recherche du CHUM, Montreal, Quebec, Canada.

William D Tolbert (WD)

Infectious Diseases Division, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.

Joseph Sodroski (J)

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA.

Marzena Pazgier (M)

Infectious Diseases Division, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.

Andrés Finzi (A)

Centre de Recherche du CHUM, Montreal, Quebec, Canada andres.finzi@umontreal.ca.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, Quebec, Canada.
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.

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Classifications MeSH