Identification performance of MALDI-ToF-MS upon mono- and bi-microbial cultures is cell number and culture proportion dependent.
Biotyping
MALDI-ToF
Mixed culture
Viable cell count
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
07
06
2019
accepted:
08
08
2019
revised:
31
07
2019
pubmed:
6
9
2019
medline:
28
11
2019
entrez:
6
9
2019
Statut:
ppublish
Résumé
Biotyping using matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) mass spectroscopy (MS) has revolutionized microbiology by allowing clinicians and scientists to rapidly identify microbes at genus and species levels. The present study extensively assesses the suitability and reliability of MALDI-ToF biotyping of 14 different aerobic and anaerobic bacterial species as pure and mixed cultures. Reliable identification at species level was possible from biomaterial of older colonies and even frozen biomaterial, although this was species dependent. Using standard instrument settings and direct application of biomaterial onto the MALDI-ToF target plates, it was determined that the cell densities necessary for completely reliable identification of pure cultures varied between 2.40 × 10
Identifiants
pubmed: 31486868
doi: 10.1007/s00216-019-02080-x
pii: 10.1007/s00216-019-02080-x
pmc: PMC6834929
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7027-7038Références
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