Metagenomic and culturomic analysis of gut microbiota dysbiosis during Clostridium difficile infection.
Adult
Aged
Aged, 80 and over
Bacteria
/ classification
Bacteroides
/ isolation & purification
Bifidobacterium
/ isolation & purification
Biological Therapy
Clostridium Infections
/ microbiology
Feces
/ microbiology
Female
Gastrointestinal Microbiome
Humans
Male
Metagenomics
Middle Aged
Molecular Typing
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 09 2019
05 09 2019
Historique:
received:
05
04
2019
accepted:
20
08
2019
entrez:
7
9
2019
pubmed:
7
9
2019
medline:
4
8
2020
Statut:
epublish
Résumé
Recently, cocktail of bacteria were proposed in order to treat Clostridium difficile infection (CDI), but these bacteriotherapies were selected more by chance than experimentation. We propose to comprehensively explore the gut microbiota of patients with CDI compared to healthy donors in order to propose a consortium of bacteria for treating C. difficile. We compared stool samples composition from 11 CDI patients and 8 healthy donors using two techniques: metagenomics, 16S V3-V4 region amplification and sequencing and culturomics, high throughout culture using six culture conditions and MALDI-TOF identification. By culturomics, we detected 170 different species in the CDI group and 275 in the control group. Bacteroidetes were significantly underrepresented in the CDI group (p = 0.007). By metagenomics, 452 different operational taxonomic units assigned to the species level were detected in the CDI group compared to 522 in the control group. By these two techniques, we selected 37 bacteria only found in control group in more than 75% of the samples and/or with high relative abundance, 10 of which have already been tested in published bacteriotherapies against CDI, and 3 of which (Bifidobacterium adolescentis, Bifidobacterium longum and Bacteroides ovatus) have been detected by these two techniques. This controlled number of bacteria could be administrated orally in a non-invasive way in order to treat CDI.
Identifiants
pubmed: 31488869
doi: 10.1038/s41598-019-49189-8
pii: 10.1038/s41598-019-49189-8
pmc: PMC6728329
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12807Références
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