Thymine DNA glycosylase recognizes the geometry alteration of minor grooves induced by 5-formylcytosine and 5-carboxylcytosine.
Journal
Chemical science
ISSN: 2041-6520
Titre abrégé: Chem Sci
Pays: England
ID NLM: 101545951
Informations de publication
Date de publication:
21 Aug 2019
21 Aug 2019
Historique:
received:
10
06
2019
accepted:
17
06
2019
entrez:
7
9
2019
pubmed:
7
9
2019
medline:
7
9
2019
Statut:
epublish
Résumé
The dynamic DNA methylation-demethylation process plays critical roles in gene expression control and cell development. The oxidation derivatives of 5-methylcytosine (5mC) generated by Tet dioxygenases in the demethylation pathway, namely 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), could impact biological functions by altering DNA properties or recognition by potential reader proteins. Hence, in addition to the fifth base 5mC, 5hmC, 5fC, and 5caC have been considered as the sixth, seventh, and eighth bases of the genome. How these modifications would alter DNA and be specifically recognized remain unclear, however. Here we report that formyl- and carboxyl-modifications on cytosine induce the geometry alteration of the DNA minor groove by solving two high-resolution structures of a dsDNA decamer containing fully symmetric 5fC and 5caC. The alterations are recognized distinctively by thymine DNA glycosylase TDG
Identifiants
pubmed: 31489163
doi: 10.1039/c9sc02807b
pii: c9sc02807b
pmc: PMC6713860
doi:
Types de publication
Journal Article
Langues
eng
Pagination
7407-7417Subventions
Organisme : NHGRI NIH HHS
ID : R01 HG006827
Pays : United States
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