Reducing sample consumption for serial crystallography using acoustic drop ejection.
acoustic dispensing
fixed targets
sample delivery
serial crystallography
Journal
Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878
Informations de publication
Date de publication:
01 Sep 2019
01 Sep 2019
Historique:
received:
22
03
2019
accepted:
28
06
2019
entrez:
7
9
2019
pubmed:
7
9
2019
medline:
7
9
2019
Statut:
ppublish
Résumé
Efficient sample delivery is an essential aspect of serial crystallography at both synchrotrons and X-ray free-electron lasers. Rastering fixed target chips through the X-ray beam is an efficient method for serial delivery from the perspectives of both sample consumption and beam time usage. Here, an approach for loading fixed targets using acoustic drop ejection is presented that does not compromise crystal quality, can reduce sample consumption by more than an order of magnitude and allows serial diffraction to be collected from a larger proportion of the crystals in the slurry.
Identifiants
pubmed: 31490175
pii: S1600577519009329
doi: 10.1107/S1600577519009329
pmc: PMC6730619
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1820-1825Informations de copyright
open access.
Références
Nat Methods. 2017 Apr;14(4):443-449
pubmed: 28250468
Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):1000-1007
pubmed: 30289410
J Struct Biol. 2015 Jul;191(1):49-58
pubmed: 26027487
Acta Crystallogr D Struct Biol. 2019 Feb 1;75(Pt 2):160-177
pubmed: 30821705
Acta Crystallogr D Struct Biol. 2018 Feb 1;74(Pt 2):85-97
pubmed: 29533234
Acta Crystallogr D Struct Biol. 2019 Feb 1;75(Pt 2):178-191
pubmed: 30821706
Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):877-894
pubmed: 30198898
J Synchrotron Radiat. 2019 Mar 1;26(Pt 2):406-412
pubmed: 30855249
Biochemistry. 2011 May 31;50(21):4399-401
pubmed: 21542590
Lab Chip. 2007 Sep;7(9):1139-45
pubmed: 17713612
Structure. 2016 Apr 5;24(4):631-640
pubmed: 26996959
Elife. 2015 Mar 17;4:
pubmed: 25781634
J Synchrotron Radiat. 2015 Nov;22(6):1372-8
pubmed: 26524301
IUCrJ. 2015 Feb 03;2(Pt 2):246-55
pubmed: 25866661