Evaluation of antimicrobial activity of chondrillasterol isolated from Vernonia adoensis (Asteraceae).
Antibacterial
Biofilms
Chondrillasterol
Vernonia adoensis
Journal
BMC complementary and alternative medicine
ISSN: 1472-6882
Titre abrégé: BMC Complement Altern Med
Pays: England
ID NLM: 101088661
Informations de publication
Date de publication:
06 Sep 2019
06 Sep 2019
Historique:
received:
14
06
2019
accepted:
27
08
2019
entrez:
8
9
2019
pubmed:
8
9
2019
medline:
31
12
2019
Statut:
epublish
Résumé
Bacteria have developed resistance to most of the current antibiotics. There is evidence suggesting that plant-derived compounds have a potential for interacting with biological processes. One of the plants commonly used in African ethnomedicine is Vernonia adoensis from the Asteraceae family. The leaves of the plant have been reported to have antimicrobial activity. Hence, the aim of this study was to isolate the bioactive compounds from the leaf extract and evaluate their antibacterial activity on Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. In addition, the effect of the isolated compound on biofilms of P. aeruginosa was determined. Isolation of phytochemicals from the leaves of V. adoensis was done using column chromatography. Preparative TLC was used to further isolate mixed compounds in the fractions. Nuclear magnetic resonance spectroscopy and mass spectrometry was used to identify the isolated pure compounds. The broth microdilution assay was carried out to evaluate the antibacterial activity of the isolated compound on P. aeruginosa, S. aureus and K. pneumoniae. Crystal violet staining technique was used to evaluate the effect of the isolated compound on biofilms of P. aeruginosa. The compound isolated from V. adoensis was identified as chondrillasterol. Chondrillasterol exhibited 25, 38 and 65% inhibition of growth on S. aureus, K. pneumoniae and P. aeruginosa respectively. At 1.6 μg/mL chondrillasterol completely disrupted mature biofilm of P. aeruginosa while at 100 μg/mL the compound completely inhibited formation of biofilms of the bacteria. Chondrillasterol isolated from V. adoensis has antibacterial properties against S. aureus, K. pneumoniae and P. aeruginosa. The compound also has biofilm inhibition and disruption activity against P. aeruginosa biofilms. Thus, the active phytochemical could be a useful template for the development of new antimicrobial agents with both antibacterial and antibiofilm activity.
Sections du résumé
BACKGROUND
BACKGROUND
Bacteria have developed resistance to most of the current antibiotics. There is evidence suggesting that plant-derived compounds have a potential for interacting with biological processes. One of the plants commonly used in African ethnomedicine is Vernonia adoensis from the Asteraceae family. The leaves of the plant have been reported to have antimicrobial activity. Hence, the aim of this study was to isolate the bioactive compounds from the leaf extract and evaluate their antibacterial activity on Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. In addition, the effect of the isolated compound on biofilms of P. aeruginosa was determined.
METHODS
METHODS
Isolation of phytochemicals from the leaves of V. adoensis was done using column chromatography. Preparative TLC was used to further isolate mixed compounds in the fractions. Nuclear magnetic resonance spectroscopy and mass spectrometry was used to identify the isolated pure compounds. The broth microdilution assay was carried out to evaluate the antibacterial activity of the isolated compound on P. aeruginosa, S. aureus and K. pneumoniae. Crystal violet staining technique was used to evaluate the effect of the isolated compound on biofilms of P. aeruginosa.
RESULTS
RESULTS
The compound isolated from V. adoensis was identified as chondrillasterol. Chondrillasterol exhibited 25, 38 and 65% inhibition of growth on S. aureus, K. pneumoniae and P. aeruginosa respectively. At 1.6 μg/mL chondrillasterol completely disrupted mature biofilm of P. aeruginosa while at 100 μg/mL the compound completely inhibited formation of biofilms of the bacteria.
CONCLUSION
CONCLUSIONS
Chondrillasterol isolated from V. adoensis has antibacterial properties against S. aureus, K. pneumoniae and P. aeruginosa. The compound also has biofilm inhibition and disruption activity against P. aeruginosa biofilms. Thus, the active phytochemical could be a useful template for the development of new antimicrobial agents with both antibacterial and antibiofilm activity.
Identifiants
pubmed: 31492140
doi: 10.1186/s12906-019-2657-7
pii: 10.1186/s12906-019-2657-7
pmc: PMC6731578
doi:
Substances chimiques
Anti-Bacterial Agents
0
Plant Extracts
0
Steroids
0
chondrillasterol
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
249Subventions
Organisme : Internationl Science Programmes
ID : IPICS-ZIM01
Organisme : Centre for Emerging and Neglected Diseasess
ID : Unversity of Zimbabwe
Organisme : International Foundation for Science
ID : IFS F/3413-03F
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