Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing.
Adaptor Proteins, Signal Transducing
/ chemistry
Amino Acid Sequence
Binding Sites
Cell Cycle Proteins
/ chemistry
Centromere
/ metabolism
Centromere Protein A
/ chemistry
Chromosomal Proteins, Non-Histone
/ chemistry
DNA-Binding Proteins
/ chemistry
HeLa Cells
Histones
/ chemistry
Humans
Molecular Chaperones
/ chemistry
Protein Binding
RNA Interference
Sequence Homology, Amino Acid
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 09 2019
06 09 2019
Historique:
received:
23
04
2019
accepted:
16
08
2019
entrez:
8
9
2019
pubmed:
8
9
2019
medline:
31
12
2019
Statut:
epublish
Résumé
Nucleosomes containing the histone H3 variant CENP-A are the epigenetic mark of centromeres, the kinetochore assembly sites required for chromosome segregation. HJURP is the CENP-A chaperone, which associates with Mis18α, Mis18β, and M18BP1 to target centromeres and deposit new CENP-A. How these proteins interact to promote CENP-A deposition remains poorly understood. Here we show that two repeats in human HJURP proposed to be functionally distinct are in fact interchangeable and bind concomitantly to the 4:2:2 Mis18α:Mis18β:M18BP1 complex without dissociating it. HJURP binds CENP-A:H4 dimers, and therefore assembly of CENP-A:H4 tetramers must be performed by two Mis18αβ:M18BP1:HJURP complexes, or by the same complex in consecutive rounds. The Mis18α N-terminal tails blockade two identical HJURP-repeat binding sites near the Mis18αβ C-terminal helices. These were identified by photo-cross-linking experiments and mutated to separate Mis18 from HJURP centromere recruitment. Our results identify molecular underpinnings of eukaryotic chromosome inheritance and shed light on how centromeres license CENP-A deposition.
Identifiants
pubmed: 31492860
doi: 10.1038/s41467-019-12019-6
pii: 10.1038/s41467-019-12019-6
pmc: PMC6731319
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Cell Cycle Proteins
0
Centromere Protein A
0
Chromosomal Proteins, Non-Histone
0
DNA-Binding Proteins
0
HJURP protein, human
0
Histones
0
MIS18A protein, human
0
Molecular Chaperones
0
OIP5 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4046Références
Bioinformatics. 2007 Nov 1;23(21):2947-8
pubmed: 17846036
Epigenetics Chromatin. 2016 May 31;9:20
pubmed: 27252782
Dev Cell. 2017 Jul 24;42(2):181-189.e3
pubmed: 28743004
J Cell Sci. 2015 Dec 15;128(24):4572-87
pubmed: 26527398
J Cell Biol. 2015 Mar 2;208(5):521-31
pubmed: 25713413
Cell. 2004 Sep 17;118(6):715-29
pubmed: 15369671
Mol Cell. 2007 Jun 22;26(6):853-65
pubmed: 17569568
Dev Cell. 2012 Jan 17;22(1):52-63
pubmed: 22169070
Dev Cell. 2016 Jun 6;37(5):413-27
pubmed: 27270040
J Mol Biol. 2010 Jan 15;395(2):361-74
pubmed: 19852970
Biophys J. 2000 Mar;78(3):1606-19
pubmed: 10692345
EMBO Rep. 2017 Jun;18(6):894-905
pubmed: 28377371
Protein Sci. 1994 Dec;3(12):2233-44
pubmed: 7538846
Protein Sci. 2018 Jan;27(1):103-111
pubmed: 28836317
Nat Biotechnol. 2012 Oct;30(10):918-20
pubmed: 23051804
J Cell Biol. 2015 Jun 22;209(6):789-801
pubmed: 26076692
J Mol Biol. 1996 Nov 29;264(2):364-76
pubmed: 8951382
Biology (Basel). 2017 Jan 24;6(1):
pubmed: 28125021
J Cell Biol. 2010 Sep 6;190(5):835-52
pubmed: 20819937
Cell. 2007 Jun 15;129(6):1153-64
pubmed: 17574026
J Am Soc Mass Spectrom. 2012 Jan;23(1):76-87
pubmed: 22038510
Chembiochem. 2011 Aug 16;12(12):1854-7
pubmed: 21678540
Mol Cell. 2017 Jan 19;65(2):231-246
pubmed: 28017591
Cell Rep. 2016 Jun 7;15(10):2127-2135
pubmed: 27239045
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1349-54
pubmed: 20080577
Cell. 2009 May 1;137(3):472-84
pubmed: 19410544
F1000Res. 2015 Aug 05;4:474
pubmed: 27127616
Dev Cell. 2014 Sep 8;30(5):496-508
pubmed: 25203206
Science. 2013 May 31;340(6136):1110-3
pubmed: 23723239
Nat Commun. 2011;2:313
pubmed: 21587230
Nature. 2016 Sep 8;537(7619):249-253
pubmed: 27580032
J Cell Biol. 2009 May 4;185(3):397-407
pubmed: 19398759
Elife. 2017 Jan 06;6:
pubmed: 28059702
Nat Rev Mol Cell Biol. 2012 Feb 15;13(3):168-82
pubmed: 22334143
Cell. 2009 May 1;137(3):485-97
pubmed: 19410545
Nucleus. 2011 Mar-Apr;2(2):146-57
pubmed: 21738837
Dev Cell. 2018 Nov 5;47(3):348-362.e7
pubmed: 30293838
Nat Rev Mol Cell Biol. 2016 Jan;17(1):16-29
pubmed: 26601620
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W349-57
pubmed: 23748958
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
J Cell Biol. 2007 Mar 12;176(6):795-805
pubmed: 17339380
EMBO J. 2013 Jul 31;32(15):2113-24
pubmed: 23771058
J Cell Sci. 2004 Dec 15;117(Pt 26):6339-53
pubmed: 15561772
Science. 2015 May 8;348(6235):699-703
pubmed: 25954010
Nucleus. 2012 Jan-Feb;3(1):101-10
pubmed: 22540025
Nat Cell Biol. 2010 Dec;12(12):1186-93
pubmed: 21102442
PLoS One. 2013 Dec 09;8(12):e82100
pubmed: 24349195
Biochemistry. 2013 Mar 12;52(10):1828-37
pubmed: 23379331
Genome Res. 2016 Sep;26(9):1178-87
pubmed: 27384170
J Cell Biol. 2015 Jul 6;210(1):11-22
pubmed: 26124289
Cell. 2009 Jun 26;137(7):1173-4
pubmed: 19563746
J Biol Chem. 2014 Mar 21;289(12):8326-36
pubmed: 24519934
Genes Dev. 2011 May 1;25(9):901-6
pubmed: 21478274
Mol Biol Cell. 2013 Apr;24(7):923-32
pubmed: 23363600
Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10571-6
pubmed: 17548816
J Cell Biol. 2011 Feb 21;192(4):569-82
pubmed: 21321101
J Cell Biol. 2007 Mar 12;176(6):757-63
pubmed: 17339379
Mol Biol Cell. 2017 Jan 1;28(1):54-64
pubmed: 27807043
Elife. 2014 Jul 15;3:e02137
pubmed: 25027692
Curr Opin Cell Biol. 2019 Jun;58:15-25
pubmed: 30654232
J Cell Biol. 2011 Jul 25;194(2):229-43
pubmed: 21768289
Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10131-6
pubmed: 24982154
Cell Rep. 2014 Jul 10;8(1):190-203
pubmed: 25001279
PLoS Biol. 2012;10(12):e1001434
pubmed: 23300376
Plant Cell. 2017 Jan;29(1):144-155
pubmed: 28062749
Cell. 2014 Jul 17;158(2):397-411
pubmed: 25036634
EMBO Rep. 2016 Apr;17(4):496-507
pubmed: 26921242
Dev Cell. 2007 Jan;12(1):17-30
pubmed: 17199038
Cell. 2011 Feb 18;144(4):471-9
pubmed: 21335232
Dev Cell. 2017 Jul 24;42(2):190-199.e10
pubmed: 28743005
Cell Rep. 2015 Apr 7;11(1):22-32
pubmed: 25843710
Mol Biol Cell. 2015 Aug 1;26(15):2742-54
pubmed: 26063729
Curr Biol. 2016 Apr 25;26(8):1110-6
pubmed: 27040782
J Biol Chem. 2019 Jan 18;294(3):968-980
pubmed: 30459232
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11020-4
pubmed: 12154230
Anal Chem. 2018 Sep 18;90(18):10990-10999
pubmed: 30074391
Mol Cell. 2016 Mar 3;61(5):774-787
pubmed: 26942680
J Cell Biol. 2011 Sep 19;194(6):855-71
pubmed: 21911481
Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9367-71
pubmed: 21606327
Genes Dev. 2007 Mar 1;21(5):497-518
pubmed: 17344412
Protein Sci. 1997 Feb;6(2):399-406
pubmed: 9041642
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Prog Mol Subcell Biol. 2017;56:165-192
pubmed: 28840237
EMBO J. 2019 Feb 15;38(4):
pubmed: 30606714
Curr Biol. 2007 Feb 6;17(3):237-43
pubmed: 17222555