A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites.
Animals
Carrier Proteins
/ genetics
Cell Line
Exocytosis
Fibroblasts
/ cytology
Host-Parasite Interactions
Humans
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Organelles
/ metabolism
Parasites
/ metabolism
Phospholipids
/ chemistry
Plasmodium falciparum
/ metabolism
Protozoan Proteins
/ genetics
Toxoplasma
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 09 2019
06 09 2019
Historique:
received:
14
06
2019
accepted:
07
08
2019
entrez:
8
9
2019
pubmed:
8
9
2019
medline:
31
12
2019
Statut:
epublish
Résumé
Members of the Apicomplexa phylum, including Plasmodium and Toxoplasma, have two types of secretory organelles (micronemes and rhoptries) whose sequential release is essential for invasion and the intracellular lifestyle of these eukaryotes. During invasion, rhoptries inject an array of invasion and virulence factors into the cytoplasm of the host cell, but the molecular mechanism mediating rhoptry exocytosis is unknown. Here we identify a set of parasite specific proteins, termed rhoptry apical surface proteins (RASP) that cap the extremity of the rhoptry. Depletion of RASP2 results in loss of rhoptry secretion and completely blocks parasite invasion and therefore parasite proliferation in both Toxoplasma and Plasmodium. Recombinant RASP2 binds charged lipids and likely contributes to assembling the machinery that docks/primes the rhoptry to the plasma membrane prior to fusion. This study provides important mechanistic insight into a parasite specific exocytic pathway, essential for the establishment of infection.
Identifiants
pubmed: 31492901
doi: 10.1038/s41467-019-11979-z
pii: 10.1038/s41467-019-11979-z
pmc: PMC6731292
doi:
Substances chimiques
Carrier Proteins
0
Phospholipids
0
Protozoan Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4041Subventions
Organisme : NIAID NIH HHS
ID : R01 AI123360
Pays : United States
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