The Spodoptera frugiperda Sf9 cell line is a heterogeneous population of rhabdovirus-infected and virus-negative cells: Isolation and characterization of cell clones containing rhabdovirus X-gene variants and virus-negative cell clones.

Animals Baculoviridae / genetics Clone Cells / cytology DNA, Intergenic / genetics Genes, Viral Genome, Viral Reverse Transcriptase Polymerase Chain Reaction Rhabdoviridae / classification Sf9 Cells / virology Spodoptera Virus Replication
Sf-rhabdovirus X-gene variants Sf-rhabdovirus-negative cell clone Sf-rhabdovirus-positive cell clone Sf9 cell line Spodoptera frugiperda

Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
10 2019
Historique:
received: 12 06 2019
revised: 31 07 2019
accepted: 01 08 2019
pubmed: 9 9 2019
medline: 9 4 2020
entrez: 9 9 2019
Statut: ppublish

Résumé

The Sf9 cell line is broadly used for manufacturing baculovirus-expressed viral vaccines. We previously reported the presence of a novel, rhabdovirus in the Sf9 cell line, which contained a unique X gene (Sf-rhabdovirus; designated as X

Identifiants

DOI: 10.1016/j.virol.2019.08.001 PMID: 31494355
pubmed: 31494355
pii: S0042-6822(19)30206-5
doi: 10.1016/j.virol.2019.08.001
pii:
doi:

Substances chimiques

DNA, Intergenic 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

125-133

Informations de copyright

Published by Elsevier Inc.

Auteurs

Hailun Ma (H)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, 20993, USA.

Subhiksha Nandakumar (S)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, 20993, USA.

Eunhae H Bae (EH)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, 20993, USA.

Pei-Ju Chin (PJ)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, 20993, USA.

Arifa S Khan (AS)

Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, 20993, USA. Electronic address: arifa.khan@fda.hhs.gov.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Virus › Virus des insectes › Baculoviridae › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Cellules › Cellules cultivées › Clones cellulaires › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › ADN intergénique › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › Gènes viraux › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › RT-PCR › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Mononegavirales › Rhabdoviridae › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Cellules Sf9 › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Lepidoptera › Papillons de nuit › Spodoptera › The Spodoptera frugiperda Sf9 cell line is a...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › The Spodoptera frugiperda Sf9 cell line is a...