Women with polycystic ovary syndrome present with altered endometrial expression of stanniocalcin-1†.
endometrial stromal cells
human endometrium
hypoxia
lifestyle intervention
polycystic ovary syndrome
primary cell culture
stanniocalcin-1
stress
Journal
Biology of reproduction
ISSN: 1529-7268
Titre abrégé: Biol Reprod
Pays: United States
ID NLM: 0207224
Informations de publication
Date de publication:
14 02 2020
14 02 2020
Historique:
received:
18
01
2019
revised:
06
08
2019
accepted:
30
08
2019
pubmed:
9
9
2019
medline:
17
6
2021
entrez:
9
9
2019
Statut:
ppublish
Résumé
Stanniocalcin-1 (STC-1) is a pro-survival factor that protects tissues against stressors, such as hypoxia and inflammation. STC-1 is co-expressed with the endometrial receptivity markers, and recently endometrial STC-1 was reported to be dysregulated in endometriosis, a condition linked with endometrial progesterone resistance and inflammation. These features are also common in the endometrium in women with polycystic ovary syndrome (PCOS), the most common endocrine disorder in women. Given that women with PCOS present with subfertility, pregnancy complications, and increased risk for endometrial cancer, we investigated endometrial STC-1 expression in affected women. Endometrial biopsy samples were obtained from women with PCOS and controls, including samples from overweight/obese women with PCOS before and after a 3-month lifestyle intervention. A total of 98 PCOS and 85 control samples were used in immunohistochemistry, reverse-transcription polymerase chain reaction, or in vitro cell culture. STC-1 expression was analyzed at different cycle phases and in endometrial stromal cells (eSCs) after steroid hormone exposure. The eSCs were also challenged with 8-bromo-cAMP and hypoxia for STC-1 expression. The findings indicate that STC-1 expression is not steroid hormone mediated although secretory-phase STC-1 expression was blunted in PCOS. Lower expression seems to be related to attenuated STC-1 response to stressors in PCOS eSCs, shown as downregulation of protein kinase A activity. The 3-month lifestyle intervention did not restore STC-1 expression in PCOS endometrium. More studies are warranted to further elucidate the mechanisms behind the altered endometrial STC-1 expression and rescue mechanism in the PCOS endometrium.
Identifiants
pubmed: 31494675
pii: 5564092
doi: 10.1093/biolre/ioz180
pmc: PMC7016287
doi:
Substances chimiques
Glycoproteins
0
teleocalcin
76687-96-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
306-315Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction.
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