Co-infection with distinct Trypanosoma cruzi strains induces an activated immune response in human monocytes.
Trypanosoma cruzi
co-infection
discrete typing units
human
monocytes
parasitemia
strains
Journal
Parasite immunology
ISSN: 1365-3024
Titre abrégé: Parasite Immunol
Pays: England
ID NLM: 7910948
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
31
01
2019
revised:
22
08
2019
accepted:
31
08
2019
pubmed:
9
9
2019
medline:
31
3
2020
entrez:
9
9
2019
Statut:
ppublish
Résumé
The aim of the study was to evaluate the immune response triggered by the first contact of human monocytes with two T cruzi strains from distinct discrete typing units (DTUs) IV and V, and whether co-infection with these strains leads to changes in monocyte immune profiles, which could in turn influence the subsequent infection outcome. We evaluated the influence of in vitro single- and co-infection with AM64 and 3253 strains on immunological characteristics of human monocytes. Single infection of monocytes with AM64 or 3253 induced opposing anti-inflammatory and inflammatory responses, respectively. Co-infection was observed in over 50% of monocytes after 15 hours of culture, but this percentage dropped ten-fold after 72 hours. Co-infection led to high monocyte activation and an increased percentage of both IL-10 and TNF. The decreased percentage of co-infected cells observed after 72 hours was associated with a decreased frequency of TNF-expressing cells. Our results show that the exacerbated response observed in co-infection with immune-polarizing strains is associated with a decreased frequency of co-infected cells, suggesting that the activated response favours parasite control. These findings may have implications for designing new Chagas disease preventive strategies.
Identifiants
pubmed: 31494949
doi: 10.1111/pim.12668
pmc: PMC6800597
mid: NIHMS1049416
doi:
Substances chimiques
IL10 protein, human
0
Tumor Necrosis Factor-alpha
0
Interleukin-10
130068-27-8
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12668Subventions
Organisme : NIAID NIH HHS
ID : R01 AI138230
Pays : United States
Organisme : NIAID NIH HHS
ID : R01AI138230
Pays : United States
Informations de copyright
© 2019 John Wiley & Sons Ltd.
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