Soft X-ray tomography: virtual sculptures from cell cultures.
Journal
Current opinion in structural biology
ISSN: 1879-033X
Titre abrégé: Curr Opin Struct Biol
Pays: England
ID NLM: 9107784
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
30
03
2019
revised:
24
06
2019
accepted:
27
06
2019
pubmed:
10
9
2019
medline:
29
7
2020
entrez:
10
9
2019
Statut:
ppublish
Résumé
Cellular complexity is represented best in high-spatial resolution, three-dimensional (3D) reconstructions. Soft X-ray tomography (SXT) generates detailed volumetric reconstructions of cells preserved in a near-to-native, frozen-hydrated state. SXT is broadly applicable and can image specimens ranging from bacteria to large mammalian cells. As a reference, we summarize light and electron microscopic methods. We then present an overview of SXT and discuss its role in cellular imaging. We detail the methods used to image biological specimens and present recent highlights that illustrate the capabilities of the technique. We conclude by discussing correlative imaging, specifically the combination of SXT and fluorescence microscopy performed on the same specimen. This correlated approach combines the structural morphology of a cell with its physiological characteristics to build a deeply informative composite view.
Identifiants
pubmed: 31495562
pii: S0959-440X(19)30053-3
doi: 10.1016/j.sbi.2019.06.012
pmc: PMC6791522
mid: NIHMS1539311
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
324-332Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103445
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
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