Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain.

Animals Brain / cytology Female Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Fluorescence, Multiphoton / methods Neurites / physiology Pyramidal Tracts / physiology Software Transfection

automated reconstruction axonal morphology long-range projections morphology database neuronal cell types neuronal connectivity projection neurons single-cell reconstruction whole brain

Journal

Cell

ISSN: 1097-4172

Titre abrégé: Cell

Pays: United States

ID NLM: 0413066

Informations de publication

Date de publication:
19 09 2019

Historique:

received: 01 02 2019

revised: 14 05 2019

accepted: 23 07 2019

pubmed: 10 9 2019

medline: 9 6 2020

entrez: 10 9 2019

Statut: ppublish

Résumé

Neuronal cell types are the nodes of neural circuits that determine the flow of information within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides an essential descriptor of cell type and reveals how individual neurons route their output across the brain. Despite the importance of morphology, few projection neurons in the mouse brain have been reconstructed in their entirety. Here we present a robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain. We used this platform to reconstruct more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons constitute more than 85 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.

Identifiants

DOI: 10.1016/j.cell.2019.07.042 PMID: 31495573 PMC: PMC6754285

pubmed: 31495573

pii: S0092-8674(19)30842-6

doi: 10.1016/j.cell.2019.07.042

pmc: PMC6754285

mid: NIHMS1044708

pii:

doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

Pagination

268-281.e13

Subventions

Organisme : NIMH NIH HHS

ID : U01 MH114824

Pays : United States

Organisme : Intramural NIH HHS

ID : Z99 MH999999

Pays : United States

Organisme : Howard Hughes Medical Institute.

Pays : International

Informations de copyright

Références

J Neurosci. 2000 Sep 15;20(18):7096-108

pubmed: 10995857

J Comp Neurol. 1975 Nov 15;164(2):233-45

pubmed: 1184784

Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6340-5

pubmed: 11972022

J Neurocytol. 2002 Mar-Jun;31(3-5):211-29

pubmed: 12815241

J Neurosci Methods. 1992 Mar;41(3):239-54

pubmed: 1381034

J Neurosci. 2004 Sep 29;24(39):8441-53

pubmed: 15456817

Neuron. 2005 Jan 20;45(2):207-21

pubmed: 15664173

PLoS Biol. 2005 Aug;3(8):e272

pubmed: 16026180

Cell. 2005 Nov 4;123(3):493-505

pubmed: 16269339

J Comp Neurol. 2006 May 10;496(2):202-13

pubmed: 16538675

Neuron. 2006 Mar 16;49(6):861-75

pubmed: 16543134

J Neurosci. 1990 Oct;10(10):3421-38

pubmed: 1698947

Brain Res. 1990 Nov 26;534(1-2):209-24

pubmed: 1705851

Nat Neurosci. 2007 May;10(5):663-8

pubmed: 17435752

Neuroscience. 2007 Sep 21;148(4):949-69

pubmed: 17707116

Brain Struct Funct. 2007 Jul;212(1):75-83

pubmed: 17717699

Neuron. 2007 Nov 21;56(4):714-25

pubmed: 18031687

Cereb Cortex. 2009 Jan;19(1):72-8

pubmed: 18403396

Nature. 2009 Feb 26;457(7233):1142-5

pubmed: 19151697

Cereb Cortex. 2009 Sep;19(9):2065-77

pubmed: 19174446

J Microsc. 2009 Jan;233(1):42-60

pubmed: 19196411

Front Neuroanat. 2010 Mar 31;4:13

pubmed: 20556241

IEEE Trans Pattern Anal Mach Intell. 2010 Dec;32(12):2262-75

pubmed: 20975122

Brain Struct Funct. 2011 Mar;216(1):1-15

pubmed: 21128083

Trends Neurosci. 2011 Jan;34(1):41-50

pubmed: 21129791

Front Neuroanat. 2010 Dec 27;4:147

pubmed: 21212837

Neuroinformatics. 2011 Sep;9(2-3):97-8

pubmed: 21279476

J Microsc. 2011 Aug;243(2):154-71

pubmed: 21361958

Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4188-93

pubmed: 21368112

Nature. 2011 Apr 14;472(7342):217-20

pubmed: 21451523

Hippocampus. 2012 Apr;22(4):693-706

pubmed: 21538658

Nat Neurosci. 2011 Jul 10;14(8):1081-8

pubmed: 21743472

Cereb Cortex. 2012 Dec;22(12):2840-57

pubmed: 22190433

J Neurosci. 2012 Apr 25;32(17):5990-9

pubmed: 22539859

J Neurosci. 2012 Jun 6;32(23):7970-85

pubmed: 22674272

Cereb Cortex. 2014 Jan;24(1):17-36

pubmed: 23010748

Nature. 2012 Dec 13;492(7428):247-51

pubmed: 23143335

BMC Bioinformatics. 2012 Nov 27;13:316

pubmed: 23181553

Nat Rev Neurosci. 2013 Apr;14(4):278-91

pubmed: 23511908

Cereb Cortex. 2015 Jan;25(1):221-35

pubmed: 23968832

Front Comput Neurosci. 2013 Nov 11;7:163

pubmed: 24273509

Cell. 2013 Dec 5;155(6):1337-50

pubmed: 24315102

Cell. 2014 Feb 27;156(5):1096-111

pubmed: 24581503

Nature. 2014 Apr 10;508(7495):207-14

pubmed: 24695228

Elife. 2014 May 07;3:e02444

pubmed: 24894464

Front Neural Circuits. 2014 Jul 10;8:76

pubmed: 25071457

Nat Neurosci. 2014 Sep;17(9):1276-85

pubmed: 25086607

Science. 2015 Apr 24;348(6233):aaa6090

pubmed: 25858977

Prog Brain Res. 2015;219:65-82

pubmed: 26072234

Neuron. 2015 Jul 15;87(2):252-6

pubmed: 26182412

Nat Protoc. 2015 Nov;10(11):1709-27

pubmed: 26448360

Nat Neurosci. 2016 Feb;19(2):335-46

pubmed: 26727548

Elife. 2016 Jan 20;5:e10566

pubmed: 26796534

Elife. 2016 Mar 21;5:e13503

pubmed: 26999799

Nat Neurosci. 2016 Apr;19(4):533-41

pubmed: 27021938

Nat Neurosci. 2016 Aug;19(8):1100-14

pubmed: 27322419

Nat Commun. 2016 Jul 04;7:12142

pubmed: 27374071

Neuroinformatics. 2016 Oct;14(4):353-67

pubmed: 27447185

Bioessays. 2016 Oct;38(10):969-76

pubmed: 27516119

Neuron. 2016 Sep 7;91(5):975-987

pubmed: 27545715

Cell. 2016 Aug 25;166(5):1308-1323.e30

pubmed: 27565351

Nature. 2017 May 11;545(7653):181-186

pubmed: 28467817

Science. 2017 May 26;356(6340):853-859

pubmed: 28546212

Nat Neurosci. 2017 Aug;20(8):1172-1179

pubmed: 28671695

Sci Rep. 2017 Jul 10;7(1):4988

pubmed: 28694478

Nat Methods. 2017 Dec;14(12):1153-1155

pubmed: 29131163

Cell Metab. 2018 Jan 9;27(1):226-236.e3

pubmed: 29320703

Cereb Cortex. 2018 May 1;28(5):1882-1897

pubmed: 29481606

Nat Commun. 2018 Mar 20;9(1):1151

pubmed: 29559622

Nature. 2018 Apr 5;556(7699):51-56

pubmed: 29590093

Cell. 2018 May 17;173(5):1280-1292.e18

pubmed: 29681453

Cell. 2018 Jul 12;174(2):363-376.e16

pubmed: 29887381

Cell. 2018 Aug 9;174(4):999-1014.e22

pubmed: 30096314

Nat Commun. 2018 Sep 3;9(1):3549

pubmed: 30177709

Elife. 2018 Oct 30;7:

pubmed: 30375971

Nature. 2018 Nov;563(7729):72-78

pubmed: 30382198

Nature. 2018 Nov;563(7729):79-84

pubmed: 30382200

Front Neuroanat. 2018 Oct 30;12:86

pubmed: 30425624

Brain Res. 1984 Jan 9;290(2):219-38

pubmed: 6198041

J Neurosci. 1983 Jul;3(7):1389-413

pubmed: 6864254

Eur J Neurosci. 1994 Oct 1;6(10):1619-32

pubmed: 7850026

Brain Res. 1994 Nov 21;664(1-2):215-9

pubmed: 7895031

J Neurophysiol. 1994 Jan;71(1):17-32

pubmed: 8158226

Somatosens Mot Res. 1993;10(1):1-16

pubmed: 8484292

J Comp Neurol. 1998 Apr 13;393(3):284-97

pubmed: 9548550

J Neurosci. 1998 Oct 15;18(20):8300-10

pubmed: 9763474

Brain Res Brain Res Rev. 1998 Dec;28(3):286-308

pubmed: 9858751