In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in
Cellobiose
Cellodextrins
Cellulolysis
Cellulose
Phosphorylase
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
2019
2019
Historique:
received:
17
06
2019
accepted:
24
08
2019
entrez:
10
9
2019
pubmed:
10
9
2019
medline:
10
9
2019
Statut:
epublish
Résumé
In anaerobic cellulolytic micro-organisms, cellulolysis results in the action of several cellulases gathered in extracellular multi-enzyme complexes called cellulosomes. Their action releases cellobiose and longer cellodextrins which are imported and further degraded in the cytosol to fuel the cells. In The three cellodextrin phosphorylases from Unexpectedly, the cellobiose phosphorylase but not the cellodextrin phosphorylases is essential for the growth of the model bacterium on cellulose. This suggests that the bacterium adopts a "short" dextrin strategy to grow on cellulose, even though the use of long cellodextrins might be more energy-saving. Our results suggest marked differences in the cellulose catabolism developed among cellulolytic bacteria, which is a result that might impact the design of future engineered strains for biomass-to-biofuel conversion.
Sections du résumé
BACKGROUND
BACKGROUND
In anaerobic cellulolytic micro-organisms, cellulolysis results in the action of several cellulases gathered in extracellular multi-enzyme complexes called cellulosomes. Their action releases cellobiose and longer cellodextrins which are imported and further degraded in the cytosol to fuel the cells. In
RESULTS
RESULTS
The three cellodextrin phosphorylases from
CONCLUSIONS
CONCLUSIONS
Unexpectedly, the cellobiose phosphorylase but not the cellodextrin phosphorylases is essential for the growth of the model bacterium on cellulose. This suggests that the bacterium adopts a "short" dextrin strategy to grow on cellulose, even though the use of long cellodextrins might be more energy-saving. Our results suggest marked differences in the cellulose catabolism developed among cellulolytic bacteria, which is a result that might impact the design of future engineered strains for biomass-to-biofuel conversion.
Identifiants
pubmed: 31497068
doi: 10.1186/s13068-019-1549-x
pii: 1549
pmc: PMC6720390
doi:
Types de publication
Journal Article
Langues
eng
Pagination
208Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interests.
Références
J Bacteriol. 2000 Sep;182(18):5172-9
pubmed: 10960102
J Mol Biol. 2000 Sep 8;302(1):205-17
pubmed: 10964570
Microbiology. 2000 Dec;146 Pt 12:3071-80
pubmed: 11101665
J Ind Microbiol Biotechnol. 2001 Nov;27(5):271-4
pubmed: 11781800
Microbiol Mol Biol Rev. 2002 Sep;66(3):506-77, table of contents
pubmed: 12209002
Appl Environ Microbiol. 2004 Mar;70(3):1563-9
pubmed: 15006779
J Bacteriol. 2004 Oct;186(19):6544-52
pubmed: 15375136
Proc Natl Acad Sci U S A. 2005 May 17;102(20):7321-5
pubmed: 15883376
J Microbiol Methods. 2007 Sep;70(3):452-64
pubmed: 17658189
BMC Bioinformatics. 2008 Jan 23;9:40
pubmed: 18215316
Nucleic Acids Res. 2009 Jan;37(Database issue):D233-8
pubmed: 18838391
Appl Biochem Biotechnol. 2010 May;161(1-8):264-73
pubmed: 19816661
Carbohydr Res. 2009 Dec 14;344(18):2468-73
pubmed: 19879558
Proteomics. 2010 Feb;10(3):541-54
pubmed: 20013800
Nat Protoc. 2010 Apr;5(4):725-38
pubmed: 20360767
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1345-9
pubmed: 22102229
Structure. 2011 Dec 7;19(12):1784-95
pubmed: 22153501
Biosci Biotechnol Biochem. 2012;76(4):812-8
pubmed: 22484959
Metab Eng. 2013 Jan;15:134-43
pubmed: 23178501
PLoS One. 2013;8(2):e56063
pubmed: 23418511
Biotechnol Biofuels. 2013 May 08;6(1):73
pubmed: 23657055
FEBS J. 2013 Sep;280(18):4463-73
pubmed: 23802549
PLoS One. 2013 Jul 25;8(7):e69360
pubmed: 23935995
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4
pubmed: 24753421
Curr Opin Biotechnol. 2014 Oct;29:171-83
pubmed: 25104562
Appl Microbiol Biotechnol. 2015 Oct;99(20):8377-90
pubmed: 26293338
Sci Rep. 2016 Mar 07;6:22770
pubmed: 26946939
PLoS One. 2016 Aug 08;11(8):e0160812
pubmed: 27501457
Sci Rep. 2017 Jul 7;7(1):4849
pubmed: 28687766
Carbohydr Res. 2017 Nov 8;451:118-132
pubmed: 28760417
Biotechnol Biofuels. 2017 Oct 30;10:250
pubmed: 29093754
Biochem J. 1983 Mar 1;209(3):803-7
pubmed: 6223623
Appl Environ Microbiol. 1983 Mar;45(3):843-9
pubmed: 6847188
Eur J Biochem. 1997 Jul 1;247(1):262-7
pubmed: 9249035
Curr Microbiol. 1997 Oct;35(4):221-7
pubmed: 9290062