Cell Death Mechanisms in Esophageal Squamous Cell Carcinoma Induced by Vesicular Stomatitis Virus Matrix Protein.
apoptosis
autophagy
oncolytic viruses
plasmids
pyroptosis
Journal
Osong public health and research perspectives
ISSN: 2210-9099
Titre abrégé: Osong Public Health Res Perspect
Pays: Korea (South)
ID NLM: 101563309
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
entrez:
10
9
2019
pubmed:
10
9
2019
medline:
10
9
2019
Statut:
ppublish
Résumé
Vesicular stomatitis virus (VSV) is under development as an oncolytic virus due to its preferential replication in cancer cells and oncolytic activity, however the viral components responsible have not yet been determined. In this study the effects of VSV wild-type (wt) and M51R-mutant matrix proteins (M51R-mMP) on apoptosis, pyroptosis, necroptosis, and autophagy pathways, in an esophagus cancer cell line (KYSE-30) were investigated. The KYSE-30 cells were transfected with pcDNA3.1 plasmids encoding wt or M51R-mMP, and apoptosis, pyroptosis, necroptosis, and autophagy were evaluated 48 and 72 hours after transfection. KYSE-30 cells transfected with VSV wt and M51R-mMPs significantly reduced cell viability to < 50% at 72 hours post-transfection. M51R-MP significantly increased the concentration of caspase-8 and caspase-9 at 48 and 72 hours post-transfection, respectively ( The results in this study indicated that VSV exerts oncolytic activity in KYSE-30 tumor cells through different cell death pathways, suggesting that M51R-mMP may potentially be used to enhance oncolysis.
Identifiants
pubmed: 31497497
doi: 10.24171/j.phrp.2019.10.4.08
pii: ophrp-10-246
pmc: PMC6711713
doi:
Types de publication
Journal Article
Langues
eng
Pagination
246-252Déclaration de conflit d'intérêts
Conflicts of Interest The authors have no conflicts of interest to declare.
Références
Mol Cell. 2000 Nov;6(5):1243-52
pubmed: 11106761
J Virol. 2003 Apr;77(8):4646-57
pubmed: 12663771
Science. 2004 Jun 4;304(5676):1500-2
pubmed: 15131264
J Virol. 2006 Jul;80(14):7028-36
pubmed: 16809308
Science. 2007 Mar 9;315(5817):1398-401
pubmed: 17272685
Virology. 2007 Jun 5;362(2):304-13
pubmed: 17292937
Mol Ther. 2007 Aug;15(8):1531-6
pubmed: 17505473
Immunity. 2007 Jul;27(1):135-44
pubmed: 17658277
Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16677-82
pubmed: 18946037
Nat Rev Microbiol. 2009 Feb;7(2):99-109
pubmed: 19148178
Immunity. 2009 Apr 17;30(4):588-98
pubmed: 19362021
Gene Ther. 2009 Jul;16(7):849-61
pubmed: 19369968
J Med Genet. 2009 Aug;46(8):497-510
pubmed: 19505876
Nat Immunol. 2010 Jan;11(1):63-9
pubmed: 19915568
J Intern Med. 2010 May;267(5):473-82
pubmed: 20433575
Nat Rev Mol Cell Biol. 2010 Oct;11(10):700-14
pubmed: 20823910
J Biol Chem. 2011 Feb 11;286(6):4517-24
pubmed: 21131352
Nat Immunol. 2011 Mar;12(3):222-30
pubmed: 21151103
J Virol. 2011 May;85(9):4167-72
pubmed: 21289120
Am J Cancer Res. 2012;2(2):214-21
pubmed: 22485198
J Biol Chem. 2012 Jun 15;287(25):21110-20
pubmed: 22556413
MBio. 2013 Feb 12;4(1):e00620-12
pubmed: 23404401
Virus Res. 2013 Sep;176(1-2):16-32
pubmed: 23796410
Nat Cell Biol. 2013 Jul;15(7):713-20
pubmed: 23817233
Int J Cell Biol. 2014;2014:502676
pubmed: 24639873
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8512-7
pubmed: 24912196
Nat Immunol. 2014 Dec;15(12):1126-33
pubmed: 25326752
Mol Oncol. 2015 Apr;9(4):806-17
pubmed: 25583602
Nature. 2015 Jan 15;517(7534):311-20
pubmed: 25592536
PLoS One. 2015 Jun 19;10(6):e0131137
pubmed: 26091335
Iran J Cancer Prev. 2016 Feb 22;9(1):e3919
pubmed: 27366311
Cancer Sci. 2016 Oct;107(10):1373-1379
pubmed: 27486853
Annu Rev Virol. 2016 Sep 29;3(1):533-553
pubmed: 27501259
Virus Res. 2017 Jan 2;227:158-170
pubmed: 27773769
Clin Transl Immunology. 2017 Jan 06;6(1):e124
pubmed: 28197335
Immunol Rev. 2017 May;277(1):76-89
pubmed: 28462525
Mol Ther. 2017 Aug 2;25(8):1900-1916
pubmed: 28527723
Biomedicines. 2017 Feb 10;5(1):
pubmed: 28536351
J Gen Virol. 2017 Nov 16;:null
pubmed: 29143726
Viruses. 2018 Feb 23;10(2):
pubmed: 29473868
Oncol Lett. 2018 Apr;15(4):4053-4060
pubmed: 29541169
Cell Death Differ. 2019 Jan;26(1):146-161
pubmed: 29666477
J Cell Biochem. 2018 Aug;119(8):6366-6382
pubmed: 29737559
Nature. 2018 Jun;558(7708):136-140
pubmed: 29849149
Ann Gastroenterol Surg. 2018 May 28;2(4):253-265
pubmed: 30003188
Front Cell Dev Biol. 2018 Oct 12;6:137
pubmed: 30370269
Int J Mol Sci. 2019 Feb 08;20(3):null
pubmed: 30744021
Proc Natl Acad Sci U S A. 2019 May 14;116(20):9714-9722
pubmed: 31048504
Transl Oncol. 2019 Jul;12(7):925-931
pubmed: 31085408
Immunity. 1998 Mar;8(3):297-303
pubmed: 9529147
Oncogene. 1998 Nov 19;17(20):2549-54
pubmed: 9840917