Volume-CLEM: a method for correlative light and electron microscopy in three dimensions.

Animals Imaging, Three-Dimensional / methods Lung / ultrastructure Mice Mice, Inbred C57BL Microscopy, Electron, Scanning / methods Microscopy, Fluorescence / methods
correlative light and electron microscopy fluorescence microscopy focused ion beam scanning electron microscopy lung tissue clearing

Journal

American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229

Informations de publication

Date de publication:
01 12 2019
Historique:
pubmed: 12 9 2019
medline: 9 4 2020
entrez: 12 9 2019
Statut: ppublish

Résumé

Generation of three-dimensional (3D) data sets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative LM and electron microscopy (EM) from 3D LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.

Identifiants

DOI: 10.1152/ajplung.00333.2019 PMID: 31508976
pubmed: 31508976
doi: 10.1152/ajplung.00333.2019
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

L778-L784

Auteurs

Jan Hegermann (J)

Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.
Research Core Unit Electron Microscopy, Hannover Medical School, Hanover, Germany.

Christoph Wrede (C)

Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.
Research Core Unit Electron Microscopy, Hannover Medical School, Hanover, Germany.

Susanne Fassbender (S)

Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.

Ronja Schliep (R)

Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.

Matthias Ochs (M)

Institute of Vegetative Anatomy, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Berlin Institute of Health, The German Center for Lung Research (DZL), Berlin, Germany.

Lars Knudsen (L)

Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.
Biomedical Research in Endstage and Obstructive Lung Research (BREATH), Member of The German Center for Lung Research (DZL), Hannover, Germany.

Christian Mühlfeld (C)

Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.
Research Core Unit Electron Microscopy, Hannover Medical School, Hanover, Germany.
Biomedical Research in Endstage and Obstructive Lung Research (BREATH), Member of The German Center for Lung Research (DZL), Hannover, Germany.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Volume-CLEM: a method for correlative light...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Volume-CLEM: a method for correlative light...
questionsmedicales.fr › Appareil respiratoire › Poumon › Volume-CLEM: a method for correlative light...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Volume-CLEM: a method for correlative light...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Volume-CLEM: a method for correlative light...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à balayage › Volume-CLEM: a method for correlative light...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Volume-CLEM: a method for correlative light...