Modulation of cardiac optogenetics by vitamin A.
cardiac optogenetics
channelrhodopsin-2
optogenetics
vitamin A
Journal
BioFactors (Oxford, England)
ISSN: 1872-8081
Titre abrégé: Biofactors
Pays: Netherlands
ID NLM: 8807441
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
05
05
2019
accepted:
16
08
2019
pubmed:
12
9
2019
medline:
18
4
2020
entrez:
12
9
2019
Statut:
ppublish
Résumé
Cardiac optogenetics is an emergent research area and refers to the delivery of light-activated proteins to excitable heart tissue and the subsequent use of light for controlling the electrical function with high spatial and temporal resolution. Channelrhodopsin-2 (ChR2) is a light-sensitive ion channel with the chromophore, all trans retinal, derived from vitamin A (all-trans-retinol; retinol). In this study, we explored whether exogenous vitamin A can be a limiting factor in the light responsiveness of cardiomyocytes-expressing ChR2. We showed that in cardiomyocytes virally transduced with ChR2 (H134R)-enhanced yellow fluorescent protein, vitamin A supplements lower than 10 μM significantly increased ChR2 expression. Adding 1 μM vitamin A changed light-induced transmembrane potential difference significantly, whereas 5 μM dramatically induced membrane depolarization and triggered intracellular calcium elevation. We concluded that vitamin A supplementation can modulate the efficiency of ChR2 and provide a complementary strategy for improving the performance of optogenetic tools.
Substances chimiques
Carrier Proteins
0
channelopsin-2, Chlamydomonas reinhardtii
0
Vitamin A
11103-57-4
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
983-990Subventions
Organisme : Medical Laser Research Center, Yara Institute Academic Center for Education, Culture and Research (ACECR)
Organisme : Research Council of University of Tehran
Informations de copyright
© 2019 International Union of Biochemistry and Molecular Biology.
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