Development and validation of a pen side test for Rift Valley fever.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
02
11
2018
accepted:
23
07
2019
entrez:
12
9
2019
pubmed:
12
9
2019
medline:
11
1
2020
Statut:
epublish
Résumé
Rift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. A first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera. The fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training.
Sections du résumé
BACKGROUND
Rift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures.
METHODOLOGY/PRINCIPAL FINDINGS
A first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera.
CONCLUSION/SIGNIFICANCE
The fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training.
Identifiants
pubmed: 31509527
doi: 10.1371/journal.pntd.0007700
pii: PNTD-D-18-01700
pmc: PMC6738586
doi:
Substances chimiques
Nucleoproteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0007700Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Rev Esp Salud Publica. 2005 Mar-Apr;79(2):177-90
pubmed: 15913053
MMWR Morb Mortal Wkly Rep. 2016 Nov 04;65(43):1200-1201
pubmed: 27811840
Vet Microbiol. 2007 Mar 31;121(1-2):29-38
pubmed: 17187944
J Med Entomol. 2010 Sep;47(5):884-9
pubmed: 20939385
PLoS Negl Trop Dis. 2016 Oct 5;10(10):e0005010
pubmed: 27706156
Clin Diagn Lab Immunol. 2002 May;9(3):713-5
pubmed: 11986283
J Clin Microbiol. 2007 Nov;45(11):3506-13
pubmed: 17804663
Vet J. 2011 Mar;187(3):402-4
pubmed: 20167519
J Clin Microbiol. 2016 Jan;54(1):49-58
pubmed: 26491176
J Egypt Public Health Assoc. 1978;53(3-4):123-8
pubmed: 572393
J Egypt Public Health Assoc. 1978;53(3-4):273-80
pubmed: 752709
Med Vet Entomol. 2017 Dec;31(4):365-372
pubmed: 28782121
Lancet Glob Health. 2016 Nov;4(11):e864-e871
pubmed: 27692776
Annu Rev Entomol. 2016;61:395-415
pubmed: 26982443
J Virol Methods. 1997 Jan;63(1-2):1-7
pubmed: 9015270
PLoS One. 2014 Oct 14;9(10):e109322
pubmed: 25313787
Appl Biochem Biotechnol. 2018 Mar;184(3):898-908
pubmed: 28918558
J Infect Dis. 2016 Oct 15;214(suppl 3):S210-S217
pubmed: 27587634
Vector Borne Zoonotic Dis. 2008 Dec;8(6):749-53
pubmed: 18620510
Arch Virol. 2018 Aug;163(8):2295-2310
pubmed: 29680923
Adv Vet Sci. 1965;10:65-127
pubmed: 5331042
J Virol Methods. 2007 Dec;146(1-2):119-24
pubmed: 17645952
PLoS Negl Trop Dis. 2016 Jun 22;10(6):e0004783
pubmed: 27331402
J Virol Methods. 2009 Apr;157(1):15-24
pubmed: 19124041
J Hyg (Lond). 1986 Oct;97(2):317-29
pubmed: 3537118
PLoS Negl Trop Dis. 2016 Jul 14;10(7):e0004827
pubmed: 27415438
J Virol Methods. 1999 Aug;81(1-2):143-54
pubmed: 10488772
PLoS Negl Trop Dis. 2012;6(4):e1623
pubmed: 22545170
PLoS Negl Trop Dis. 2015 Jun 30;9(6):e0003902
pubmed: 26125560
Trop Anim Health Prod. 1981 Nov;13(4):185-8
pubmed: 7344184
J Clin Microbiol. 2001 Dec;39(12):4456-61
pubmed: 11724861
J Virol Methods. 2013 Nov;193(2):426-31
pubmed: 23850696
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9405-10
pubmed: 15197258
Lancet Infect Dis. 2016 Dec;16(12):1319-1320
pubmed: 27998581
J Clin Microbiol. 2002 Jul;40(7):2323-30
pubmed: 12089242
J Gen Virol. 2014 Feb;95(Pt 2):292-300
pubmed: 24096318
PLoS Negl Trop Dis. 2017 Nov 20;11(11):e0006075
pubmed: 29155823
PLoS Negl Trop Dis. 2014 Jul 31;8(7):e3045
pubmed: 25078616
New Microbes New Infect. 2015 Oct 28;9:1-7
pubmed: 26740887
Transbound Emerg Dis. 2018 Feb;65(1):197-204
pubmed: 28387065
Virol J. 2008 Jul 18;5:82
pubmed: 18638365
Am J Trop Med Hyg. 2015 Apr;92(4):722-9
pubmed: 25732680