Effects of preservation methodology on stable isotope compositions of sea stars.
Journal
Rapid communications in mass spectrometry : RCM
ISSN: 1097-0231
Titre abrégé: Rapid Commun Mass Spectrom
Pays: England
ID NLM: 8802365
Informations de publication
Date de publication:
30 Jan 2020
30 Jan 2020
Historique:
received:
19
07
2019
revised:
06
09
2019
accepted:
07
09
2019
pubmed:
12
9
2019
medline:
18
9
2020
entrez:
12
9
2019
Statut:
ppublish
Résumé
Stable isotope analysis is used to investigate the trophic ecology of organisms and, in order to use samples from archived collections, it is important to know whether preservation methods alter the results. This study investigates the long-term effects of four preservation methods on isotopic compositions and isotopic niche parameters of sea stars. We assessed the effects of preservation method (freezing, drying, formaldehyde, ethanol) and duration (0, 1, 3, 6, 9, 12, 24 months) on the stable isotope ratios of carbon, nitrogen and sulfur of sea star tissues. Isotopic ratios were measured using continuous-flow elemental analysis and isotope ratio mass spectrometry. We also monitored the evolution of commonly used ecological metrics (isotopic niche parameters) throughout the experiment. Clear changes in δ Overall, these results show that preservation may affect the stable isotope ratios of sea stars. Correction factors can be used to mitigate the effects of the preservation method on stable isotope ratios. Isotopic niche parameters are overall unchanged. Consequently, in most cases, museum samples are suitable for calculation of isotopic niche parameters.
Substances chimiques
Carbon Isotopes
0
Nitrogen Isotopes
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e8589Subventions
Organisme : Belgian Science Policy Office (BELSPO)
ID : BR/154/A1/RECTO
Organisme : Belgian Science Policy Office (BELSPO)
ID : BR/132/A1/vERSO
Informations de copyright
© 2019 John Wiley & Sons, Ltd.
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