Multicenter Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: a Report from the Clinical and Laboratory Standards Institute.


Journal

Journal of clinical microbiology
ISSN: 1098-660X
Titre abrégé: J Clin Microbiol
Pays: United States
ID NLM: 7505564

Informations de publication

Date de publication:
11 2019
Historique:
received: 01 08 2019
accepted: 05 09 2019
pubmed: 13 9 2019
medline: 25 7 2020
entrez: 13 9 2019
Statut: epublish

Résumé

Susceptibility testing of the polymyxins (colistin and polymyxin B) is challenging for clinical laboratories. The Clinical and Laboratory Standards Institute (CLSI) Antimicrobial Susceptibility Testing Subcommittee evaluated two methods to enable accurate testing of these agents. These methods were a colistin broth disk elution (CBDE) and a colistin agar test (CAT), the latter of which was evaluated using two inoculum volumes, 1 μl (CAT-1) and 10 μl (CAT-10). The methods were evaluated using a collection of 270 isolates of

Identifiants

pubmed: 31511331
pii: JCM.01269-19
doi: 10.1128/JCM.01269-19
pmc: PMC6813006
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Agar 9002-18-0
Colistin Z67X93HJG1

Types de publication

Evaluation Study Journal Article Multicenter Study

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2019 American Society for Microbiology.

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Auteurs

Romney M Humphries (RM)

Accelerate Diagnostics, Tucson Arizona, USA rhumphries@axdx.com.
Department of Pathology, University of Arizona, Tucson, Arizona, USA.

Daniel A Green (DA)

Clinical Microbiology Laboratory, Department of Pathology & Cell Biology, Columbia University Irving Medical Center, New York, New York, USA.

Audrey N Schuetz (AN)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.

Yehudit Bergman (Y)

Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Shawna Lewis (S)

Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Rebecca Yee (R)

Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Stephania Stump (S)

Division of Infectious Diseases, Columbia University Irving Medical Center, New York, New York, USA.

Mabel Lopez (M)

Clinical Microbiology Laboratory, Department of Pathology & Cell Biology, Columbia University Irving Medical Center, New York, New York, USA.

Nenad Macesic (N)

Division of Infectious Diseases, Columbia University Irving Medical Center, New York, New York, USA.

Anne-Catrin Uhlemann (AC)

Division of Infectious Diseases, Columbia University Irving Medical Center, New York, New York, USA.

Peggy Kohner (P)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

Nicolynn Cole (N)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

Patricia J Simner (PJ)

Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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Classifications MeSH