ApoF knockdown increases cholesteryl ester transfer to LDL and impairs cholesterol clearance in fat-fed hamsters.
Animals
Apoproteins
/ deficiency
Cholesterol Ester Transfer Proteins
/ metabolism
Cholesterol Esters
/ metabolism
Cholesterol, LDL
/ metabolism
Cricetinae
Diet
Female
Gene Expression Regulation
/ genetics
Gene Knockdown Techniques
HEK293 Cells
Humans
Liver
/ metabolism
Male
RNA, Small Interfering
/ genetics
apolipoprotein F
cholesteryl ester transfer protein
lipoproteins
low density lipoprotein
reverse cholesterol transport
siRNA interference
small interfering ribonucleic acid
Journal
Journal of lipid research
ISSN: 1539-7262
Titre abrégé: J Lipid Res
Pays: United States
ID NLM: 0376606
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
31
05
2019
revised:
10
09
2019
pubmed:
13
9
2019
medline:
10
7
2020
entrez:
13
9
2019
Statut:
ppublish
Résumé
Cholesteryl ester transfer protein (CETP) regulates intravascular lipoprotein metabolism. In vitro studies indicate that ApoF alters CETP function by inhibiting its activity with LDL. To explore in vivo the complexities driving ApoF's effects on CETP, we developed a siRNA-based hamster model of ApoF knockdown. In both male and female hamsters on chow- or fat-fed diets, we measured lipoprotein levels and composition, determined CETP-mediated transfer of cholesteryl esters (CEs) between lipoproteins, and quantified reverse cholesterol transport (RCT). We found that apoF knockdown in chow-fed hamsters had no effect on lipoprotein levels or composition, but these ApoF-deficient lipoproteins supported 50-100% higher LDL CETP activity in vitro. ApoF knockdown in fat-fed male hamsters created a phenotype in which endogenous CETP-mediated CE transfer from HDL to LDL increased up to 2-fold, LDL cholesterol increased 40%, HDL declined 25%, LDL and HDL lipid compositions were altered, and hepatic
Identifiants
pubmed: 31511396
pii: S0022-2275(20)32285-9
doi: 10.1194/jlr.RA119000171
pmc: PMC6824501
doi:
Substances chimiques
Apoproteins
0
Cholesterol Ester Transfer Proteins
0
Cholesterol Esters
0
Cholesterol, LDL
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1868-1879Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL130041
Pays : United States
Informations de copyright
Copyright © 2019 Morton et al.
Références
J Clin Invest. 2007 Jan;117(1):153-64
pubmed: 17200715
IUBMB Life. 2011 Apr;63(4):248-57
pubmed: 21488146
J Lipid Res. 1995 Jul;36(7):1567-85
pubmed: 7595080
PLoS One. 2012;7(2):e31616
pubmed: 22363685
J Clin Invest. 1992 Aug;90(2):505-10
pubmed: 1644921
J Lipid Res. 2002 Aug;43(8):1264-74
pubmed: 12177170
J Lipid Res. 1994 Jun;35(6):982-92
pubmed: 8077854
J Clin Invest. 1991 Dec;88(6):2059-66
pubmed: 1752964
J Lipid Res. 2008 Jul;49(7):1529-37
pubmed: 18369235
J Lipid Res. 2004 Sep;45(9):1594-607
pubmed: 15145983
J Lipid Res. 2009 Apr;50(4):676-84
pubmed: 19008550
Arterioscler Thromb. 1993 Dec;13(12):1843-51
pubmed: 8241106
Arterioscler Thromb Vasc Biol. 1997 Nov;17(11):3041-8
pubmed: 9409291
J Biol Chem. 1994 Nov 25;269(47):29588-91
pubmed: 7961945
Anal Biochem. 1977 Dec;83(2):346-56
pubmed: 603028
J Lipid Res. 2000 Jun;41(6):1020-6
pubmed: 10828095
J Lipid Res. 2003 Dec;44(12):2287-96
pubmed: 12951364
Arterioscler Thromb Vasc Biol. 2006 Apr;26(4):706-15
pubmed: 16439711
J Lipid Res. 1994 May;35(5):836-47
pubmed: 8071606
J Lipid Res. 2014 Feb;55(2):258-65
pubmed: 24293641
J Lipid Res. 1992 Jan;33(1):77-88
pubmed: 1552235
J Lipid Res. 1994 May;35(5):793-802
pubmed: 8071602
J Lipid Res. 2008 Jan;49(1):127-35
pubmed: 17901467
J Biol Chem. 1983 Oct 10;258(19):11751-7
pubmed: 6619141
Methods Enzymol. 1986;128:417-31
pubmed: 3724517
J Lipid Res. 2009 Sep;50(9):1842-51
pubmed: 19417222
FEBS Open Bio. 2014 Sep 02;4:741-5
pubmed: 25349778
Biochim Biophys Acta. 1984 Oct 4;795(3):473-80
pubmed: 6477956
J Lipid Res. 2003 Jul;44(7):1364-72
pubmed: 12730298
Atherosclerosis. 2014 Mar;233(1):234-41
pubmed: 24529150
Biochim Biophys Acta. 1981 Jan 26;663(1):350-5
pubmed: 6783110
Metabolism. 1995 Oct;44(10):1326-31
pubmed: 7476293
J Biol Chem. 1988 Sep 5;263(25):12235-41
pubmed: 3410842
J Clin Invest. 1989 Jul;84(1):119-28
pubmed: 2738148
Cell. 1997 May 2;89(3):331-40
pubmed: 9150132
J Biol Chem. 2003 Oct 17;278(42):40859-66
pubmed: 12907677
Arterioscler Thromb Vasc Biol. 2009 Jan;29(1):40-6
pubmed: 19008531
J Clin Invest. 1955 Sep;34(9):1345-53
pubmed: 13252080
J Lipid Res. 1986 May;27(5):523-9
pubmed: 3734635
Biochim Biophys Acta. 2000 Jul 19;1486(2-3):275-84
pubmed: 10903478
Arterioscler Thromb. 1991 Jul-Aug;11(4):797-804
pubmed: 2065034
J Lipid Res. 1990 Sep;31(9):1559-67
pubmed: 2246609
J Lipid Res. 2009 Apr;50 Suppl:S189-94
pubmed: 19064999
J Biol Chem. 1981 Dec 10;256(23):11992-5
pubmed: 7298638
J Biol Chem. 1999 Jan 15;274(3):1814-20
pubmed: 9880564
J Biol Chem. 1959 Mar;234(3):466-8
pubmed: 13641241
Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):1819-28
pubmed: 7583561
J Biol Chem. 1991 Mar 5;266(7):4631-9
pubmed: 1999438
Curr Opin Lipidol. 1999 Aug;10(4):321-7
pubmed: 10482135
J Lipid Res. 1993 Aug;34(8):1255-74
pubmed: 8409761
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Arterioscler Thromb Vasc Biol. 1999 Mar;19(3):718-26
pubmed: 10073979
J Biol Chem. 2005 Nov 11;280(45):38108-16
pubmed: 16159884
J Clin Invest. 1995 Oct;96(4):2071-4
pubmed: 7560101
J Lipid Res. 2011 Dec;52(12):2262-71
pubmed: 21937674
Curr Opin Lipidol. 2008 Jun;19(3):307-13
pubmed: 18460924
J Biol Chem. 2002 Aug 30;277(35):31354-63
pubmed: 12070157
J Biol Chem. 2000 Dec 1;275(48):37504-9
pubmed: 10978346
J Biol Chem. 1994 Feb 4;269(5):3189-97
pubmed: 8106353
Biochim Biophys Acta. 1978 Sep 28;530(3):428-38
pubmed: 212110
J Clin Invest. 1994 Dec;94(6):2457-67
pubmed: 7989603
J Lipid Res. 1993 Jun;34(6):943-59
pubmed: 8354960
Biochim Biophys Acta. 1996 Nov 22;1304(2):145-60
pubmed: 8954138