Staphylococcus aureus Lpl protein triggers human host cell invasion via activation of Hsp90 receptor.
F-actin
Hsp90 receptor
Lpl
Staphylococcus aureus
invasion
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
11
06
2019
revised:
02
08
2019
accepted:
26
08
2019
pubmed:
14
9
2019
medline:
10
4
2021
entrez:
14
9
2019
Statut:
ppublish
Résumé
Staphylococcus aureus is a facultative intracellular pathogen. Recently, it has been shown that the protein part of the lipoprotein-like lipoproteins (Lpls), encoded by the lpl cluster comprising of 10 lpls paralogue genes, increases pathogenicity, delays the G2/M phase transition, and also triggers host cell invasion. Here, we show that a recombinant Lpl1 protein without the lipid moiety binds directly to the isoforms of the human heat shock proteins Hsp90α and Hsp90ß. Synthetic peptides covering the Lpl1 sequence caused a twofold to fivefold increase of S. aureus invasion in HaCaT cells. Antibodies against Hsp90 decrease S. aureus invasion in HaCaT cells and in primary human keratinocytes. Additionally, inhibition of ATPase function of Hsp90 or silencing Hsp90α expression by siRNA also decreased the S. aureus invasion in HaCaT cells. Although the Hsp90ß is constitutively expressed, the Hsp90α isoform is heat-inducible and appears to play a major role in Lpl1 interaction. Pre-incubation of HaCaT cells at 39°C increased both the Hsp90α expression and S. aureus invasion. Lpl1-Hsp90 interaction induces F-actin formation, thus, triggering an endocytosis-like internalisation. Here, we uncovered a new host cell invasion principle on the basis of Lpl-Hsp90 interaction.
Substances chimiques
Actins
0
Bacterial Proteins
0
HSP90 Heat-Shock Proteins
0
HSP90AA2P protein, human
0
HSP90AB1 protein, human
0
Lipoproteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13111Informations de copyright
© 2019 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.
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