Expression, purification, and cytotoxic evaluation of IL24-BR2 fusion protein.
BR2
Cytotoxicity
Fusion protein
IL24
IL24-BR2
Journal
Research in pharmaceutical sciences
ISSN: 1735-5362
Titre abrégé: Res Pharm Sci
Pays: Iran
ID NLM: 101516968
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
entrez:
14
9
2019
pubmed:
14
9
2019
medline:
14
9
2019
Statut:
ppublish
Résumé
Interleukin (IL) 24 is a pro-inflammatory and tumor suppressor cytokine capable of inducing selective apoptosis in various cancer cells. BR2, on the other hand, is an anti-microbial peptide with selective penetrability to the cancer cells. In this study, we aimed to produce and purify a fusion protein containing IL24 as the toxic moiety fused to BR2, as targeting moiety, and then to evaluate its cytotoxic activities. For this purpose, the coding sequence of IL24-BR2 fusion protein and IL24 were cloned into the pET28a vector and used to transform
Identifiants
pubmed: 31516508
doi: 10.4103/1735-5362.263556
pii: RPS-14-320
pmc: PMC6714114
doi:
Types de publication
Journal Article
Langues
eng
Pagination
320-328Références
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