An insight into analysis and elimination of plasmids encoding metallo-β-lactamases in Pseudomonas aeruginosa.


Journal

Journal of global antimicrobial resistance
ISSN: 2213-7173
Titre abrégé: J Glob Antimicrob Resist
Pays: Netherlands
ID NLM: 101622459

Informations de publication

Date de publication:
06 2020
Historique:
received: 28 07 2018
revised: 22 08 2019
accepted: 02 09 2019
pubmed: 14 9 2019
medline: 7 4 2021
entrez: 14 9 2019
Statut: ppublish

Résumé

The aim of this study was to characterise metallo-β-lactamase (MBL)-harbouring plasmids, their change in copy number in respect to different antibiotic pressure, and the efficiency of different curing agents in eliminating these resistance plasmids from nosocomial Pseudomonas aeruginosa isolates. Plasmids were extracted from four isolates harbouring bla Conjugatively transferable MBL genes (bla The spread of multidrug resistance plasmids has been noted as a key factor associated with increasing carbapenem resistance. Successful curing of resistance plasmids can reverse the bacterial phenotype back to susceptible. This study revealed that different antibiotic pressure induces a change in copy number of MBL-encoding plasmids. SDS can be successfully used as an eliminating agent for these resistance determinants, although therapeutic application of this agent is not possible due to its high toxicity and mutagenic nature.

Identifiants

pubmed: 31518725
pii: S2213-7165(19)30234-6
doi: 10.1016/j.jgar.2019.09.002
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Sodium Dodecyl Sulfate 368GB5141J
Imipenem 71OTZ9ZE0A
beta-Lactamases EC 3.5.2.6
Meropenem FV9J3JU8B1
Aztreonam G2B4VE5GH8
Ertapenem G32F6EID2H
Cefotaxime N2GI8B1GK7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3-7

Informations de copyright

Copyright © 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved.

Auteurs

Deepjyoti Paul (D)

Department of Microbiology, Assam University, Silchar, India.

Debadatta Dhar Chanda (DD)

Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.

Atanu Chakravarty (A)

Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.

Amitabha Bhattacharjee (A)

Department of Microbiology, Assam University, Silchar, India. Electronic address: ab0404@gmail.com.

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Classifications MeSH