Tissue Harvesting for Adoptive Tumor Infiltrating Lymphocyte Therapy in Metastatic Melanoma.


Journal

Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988

Informations de publication

Date de publication:
Sep 2019
Historique:
received: 06 08 2019
revised: 16 08 2019
accepted: 17 08 2019
entrez: 15 9 2019
pubmed: 15 9 2019
medline: 27 9 2019
Statut: ppublish

Résumé

Adoptive transfer of tumor-infiltrating lymphocytes (TILs) combined with non-myeloablative chemotherapy (NMA) has been shown to prolong survival in patients with metastatic disease. Tissue harvesting was performed form a variety of sites. TILs were isolated, expanded and infused with bolus high-dose IL-2. Between 2008 and 2018, 242 lesions were resected for TILs harvesting from a range of sites form 196 patients without mortality and with minimal morbidity. Of those harvested, 75 were unable to complete therapy because of clinical deterioration during the wait period. Of 121 evaluable treated patients, there was no effect of metastatic site biopsied on the mean fold TIL expansion. Those receiving prior ipilimumab had a higher TIL fold expansion but a lower TIL fold expansion than those exposed to anti-PD1 therapy. Harvesting may be safely performed with successful TIL expansion from most sites. Prior check point inhibitory immunotherapy may potentially influence TIL fold expansion.

Sections du résumé

BACKGROUND/AIM OBJECTIVE
Adoptive transfer of tumor-infiltrating lymphocytes (TILs) combined with non-myeloablative chemotherapy (NMA) has been shown to prolong survival in patients with metastatic disease.
MATERIALS AND METHODS METHODS
Tissue harvesting was performed form a variety of sites. TILs were isolated, expanded and infused with bolus high-dose IL-2.
RESULTS RESULTS
Between 2008 and 2018, 242 lesions were resected for TILs harvesting from a range of sites form 196 patients without mortality and with minimal morbidity. Of those harvested, 75 were unable to complete therapy because of clinical deterioration during the wait period. Of 121 evaluable treated patients, there was no effect of metastatic site biopsied on the mean fold TIL expansion. Those receiving prior ipilimumab had a higher TIL fold expansion but a lower TIL fold expansion than those exposed to anti-PD1 therapy.
CONCLUSION CONCLUSIONS
Harvesting may be safely performed with successful TIL expansion from most sites. Prior check point inhibitory immunotherapy may potentially influence TIL fold expansion.

Identifiants

pubmed: 31519606
pii: 39/9/4995
doi: 10.21873/anticanres.13689
doi:

Substances chimiques

Antineoplastic Agents, Immunological 0
Biomarkers, Tumor 0
PDCD1 protein, human 0
Programmed Cell Death 1 Receptor 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4995-5001

Informations de copyright

Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Auteurs

Douglas Zippel (D)

Department of Surgery C & Surgical Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel dov.zippel@sheba.health.gov.il.
Ella Lemelbaum Institute for Immuno Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Surgery, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Orli Friedman-Eldar (O)

Department of Surgery C & Surgical Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Ella Lemelbaum Institute for Immuno Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Surgery, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Shlomi Rayman (S)

Department of Surgery C & Surgical Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.

David Hazzan (D)

Department of Surgery C & Surgical Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Surgery, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Aviram Nissan (A)

Department of Surgery C & Surgical Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Surgery, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Gal Schtrechman (G)

Department of Surgery C & Surgical Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.

Gal Markel (G)

Ella Lemelbaum Institute for Immuno Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Jacob Schachter (J)

Ella Lemelbaum Institute for Immuno Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Orit Itzhaki (O)

Ella Lemelbaum Institute for Immuno Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Michal J Besser (MJ)

Ella Lemelbaum Institute for Immuno Oncology, Chaim Sheba Medical Center, Ramat Gan, Israel.
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

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Classifications MeSH