Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein.
Animals
Antibodies, Monoclonal
/ immunology
Antibodies, Viral
/ immunology
Antigens, Viral
/ immunology
Camelids, New World
/ virology
Epitopes
/ immunology
Formaldehyde
Humans
Immunohistochemistry
Middle East Respiratory Syndrome Coronavirus
/ chemistry
Nose
/ virology
Paraffin Embedding
Prospective Studies
Spike Glycoprotein, Coronavirus
/ immunology
Immunohistochemistry
Middle East respiratory syndrome coronavirus
Monoclonal human antibodies
Spike protein
Journal
Veterinary immunology and immunopathology
ISSN: 1873-2534
Titre abrégé: Vet Immunol Immunopathol
Pays: Netherlands
ID NLM: 8002006
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
10
05
2019
revised:
02
09
2019
accepted:
08
09
2019
pubmed:
19
9
2019
medline:
26
3
2020
entrez:
19
9
2019
Statut:
ppublish
Résumé
Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.
Identifiants
pubmed: 31526954
pii: S0165-2427(19)30171-0
doi: 10.1016/j.vetimm.2019.109939
pmc: PMC7112921
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Viral
0
Antigens, Viral
0
Epitopes
0
Spike Glycoprotein, Coronavirus
0
Formaldehyde
1HG84L3525
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109939Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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