Bifunctional Bioactive Polymer Surfaces with Micrometer and Submicrometer-sized Structure: The Effects of Structure Spacing and Elastic Modulus on Bioactivity.
chemical surface modification
colloidal lithography
microcontact printing
structured polymer surfaces
surface-cell interactions
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
16 Sep 2019
16 Sep 2019
Historique:
received:
01
08
2019
revised:
16
08
2019
accepted:
27
08
2019
entrez:
19
9
2019
pubmed:
19
9
2019
medline:
6
2
2020
Statut:
epublish
Résumé
This study presents a comparison of two types of bifunctional structured surface that were made from the same polymer -- an antimicrobial polycation (a synthetic mimic of an antimicrobial peptide, SMAMP) and a protein-repellent polyzwitterion (poly(sulfobetaines), PSB). The first type of bifunctional surface was fabricated by a colloidal lithography (CL) based process where the two polymers were immobilized sequentially onto pre-structured surfaces with a chemical contrast (gold on silicon). This enabled site-selective covalent attachment. The CL materials had a spacing ranging from 200 nm to 2 µm. The second type of structured surface (spacing: 1 - 8.5 µm) was fabricated using a microcontact printing (µCP) process where SMAMP patches were printed onto a PSB network, so that 3D surface features were obtained. The thus obtained materials were studied by quantitative nanomechanical measurements using atomic force microscopy (QNM-AFM). The different architectures led to different local elastic moduli at the polymer-air interface, where the CL surfaces were much stiffer (Derjaguin-Muller-Toporov (DMT) modulus = 20 ± 0.8 GPa) compared to the structured 3D networks obtained by µCP (DMT modulus = 42 ± 1.1 MPa). The effects of the surface topology and stiffness on the antimicrobial activity against
Identifiants
pubmed: 31527527
pii: molecules24183371
doi: 10.3390/molecules24183371
pmc: PMC6767307
pii:
doi:
Substances chimiques
Anti-Infective Agents
0
Biopolymers
0
Colloids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg
ID : GenMik II Graduate School
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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