Status of serine tyrosine kinase at germline and expressional levels in asthma patients.
ELISA
Expression
SSCP
Spleen tyrosine kinase
Journal
Molecular biology research communications
ISSN: 2345-2005
Titre abrégé: Mol Biol Res Commun
Pays: Iran
ID NLM: 101613459
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
entrez:
19
9
2019
pubmed:
19
9
2019
medline:
19
9
2019
Statut:
ppublish
Résumé
Asthma is a disease marked by inflammation of airways with an increasing incidence rate worldwide especially among Asian population. Spleen tyrosine kinase (Syk) is known to be involved in regulation of such inflammation response and thereby rendering its inevitable importance among asthma patients. DNA extraction followed by PCR and sequencing was performed for genomic analysis, mRNA analysis was done by RT PCR whereas Western blot and ELISA was used for protein study. Image J and UNAFOLD were also used for Bioinformatics analysis.The mean age of patients and controls were 31.1±9.3 and 30.4±6.1 years respectively. Results of sequencing showed nonsense exonic mutations in exon 3 at g.25710G>A and g.25722G
Identifiants
pubmed: 31531378
doi: 10.22099/mbrc.2019.33040.1394
pmc: PMC6715266
doi:
Types de publication
Journal Article
Langues
eng
Pagination
69-77Déclaration de conflit d'intérêts
All authors declare that no conflict of interest exists.
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