Altered muscle electrical tissue properties in a mouse model of premature aging.
Aging, Premature
/ pathology
Animals
Cell Size
DNA Polymerase gamma
/ genetics
DNA, Mitochondrial
/ genetics
Disease Models, Animal
Electric Impedance
Gene Knock-In Techniques
Mice
Muscle Fibers, Skeletal
/ drug effects
Muscle, Skeletal
/ drug effects
Mutation
Myography
/ methods
Nicotinamide Mononucleotide
/ pharmacology
Aging
Impedance
Mitochondrial disorder
Muscle
Myofiber
Nicotinamide mononucleotide
Journal
Muscle & nerve
ISSN: 1097-4598
Titre abrégé: Muscle Nerve
Pays: United States
ID NLM: 7803146
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
16
05
2019
revised:
07
09
2019
accepted:
11
09
2019
pubmed:
19
9
2019
medline:
14
1
2020
entrez:
19
9
2019
Statut:
ppublish
Résumé
Improved methods are needed to detect and quantify age-related muscle change. In this study we assessed the electrical properties of muscle impacted by acquired mitochondrial DNA mutations via the PolG mouse, which exhibits typical age-associated features, and the impact of a potential therapy, nicotinamide mononucleotide (NMN). The gastrocnemii of 24 PolG and 30 wild-type (WT) mice (8 PolG and 17 WT treated with NMN) were studied in an electrical impedance-measuring cell. Conductivity and relative permittivity were determined from the impedance data. Myofiber cross-sectional area (CSA) was quantified histologically. Untreated PolG mice demonstrated alterations in several impedance features, including 50-kHz relative permittivity and center frequency. A potential effect of NMN was also observed in these parameters in PolG but not WT animals. Impedance values correlated with myofiber CSA. Electrical impedance is sensitive to myofiber features considered characteristic of aging and to the impact of a potential therapy.
Identifiants
pubmed: 31531861
doi: 10.1002/mus.26714
pmc: PMC7718596
mid: NIHMS1648870
doi:
Substances chimiques
DNA, Mitochondrial
0
Nicotinamide Mononucleotide
1094-61-7
DNA Polymerase gamma
EC 2.7.7.7
Polg protein, mouse
EC 2.7.7.7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
801-810Subventions
Organisme : NINDS NIH HHS
ID : R01 NS091159
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS094840
Pays : United States
Informations de copyright
© 2019 Wiley Periodicals, Inc.
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