The TGFB2-AS1 lncRNA Regulates TGF-β Signaling by Modulating Corepressor Activity.
EED
EZH2
PRC2
SUZ12
Smad
TGF-β
corepressor
lncRNA
signal transduction
transcription
tumor suppression
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
17 Sep 2019
17 Sep 2019
Historique:
received:
11
10
2018
revised:
08
05
2019
accepted:
05
08
2019
entrez:
19
9
2019
pubmed:
19
9
2019
medline:
12
9
2020
Statut:
ppublish
Résumé
Molecular processes involving lncRNAs regulate cell function. By applying transcriptomics, we identify lncRNAs whose expression is regulated by transforming growth factor β (TGF-β). Upon silencing individual lncRNAs, we identify several that regulate TGF-β signaling. Among these lncRNAs, TGFB2-antisense RNA1 (TGFB2-AS1) is induced by TGF-β through Smad and protein kinase pathways and resides in the nucleus. Depleting TGFB2-AS1 enhances TGF-β/Smad-mediated transcription and expression of hallmark TGF-β-target genes. Increased dose of TGFB2-AS1 reduces expression of these genes, attenuates TGF-β-induced cell growth arrest, and alters BMP and Wnt pathway gene profiles. Mechanistically, TGFB2-AS1, mainly via its 3' terminal region, binds to the EED adaptor of the Polycomb repressor complex 2 (PRC2), promoting repressive histone H3K27me
Identifiants
pubmed: 31533040
pii: S2211-1247(19)31062-9
doi: 10.1016/j.celrep.2019.08.028
pmc: PMC6859500
pii:
doi:
Substances chimiques
RNA, Antisense
0
RNA, Long Noncoding
0
Transforming Growth Factor beta
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3182-3198.e11Subventions
Organisme : European Research Council
ID : 787472
Pays : International
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
Références
Nat Rev Mol Cell Biol. 2018 Jul;19(7):419-435
pubmed: 29643418
Nat Rev Mol Cell Biol. 2018 Apr;19(4):262-274
pubmed: 29209056
Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11667-72
pubmed: 19571010
Nat Commun. 2014;5:3127
pubmed: 24457600
Cancer Res. 2019 Jun 1;79(11):2821-2838
pubmed: 30952633
Nat Rev Nephrol. 2016 Jun;12(6):325-38
pubmed: 27108839
J Biol Chem. 2015 Mar 13;290(11):6857-67
pubmed: 25605728
RNA. 2015 Dec;21(12):2007-22
pubmed: 26574518
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W70-4
pubmed: 18424795
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
J Biochem. 2015 Nov;158(5):373-84
pubmed: 26002960
Cancer Cell. 2014 May 12;25(5):666-81
pubmed: 24768205
Mol Cell Biol. 2004 May;24(10):4241-54
pubmed: 15121845
Oncotarget. 2017 May 17;8(46):80223-80234
pubmed: 29113297
Cancer Lett. 2016 Jan 28;370(2):296-301
pubmed: 26577807
Nat Commun. 2015 Jul 24;6:7743
pubmed: 26205790
Front Genet. 2015 Feb 27;6:72
pubmed: 25774169
Nucleic Acids Res. 2019 Jan 8;47(D1):D135-D139
pubmed: 30371849
Nat Commun. 2017 Sep 22;8(1):672
pubmed: 28939884
J Biol Chem. 2017 Aug 25;292(34):14270-14278
pubmed: 28659340
Cancer Sci. 2018 Jul;109(7):2211-2220
pubmed: 29722104
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12747-52
pubmed: 16908841
Annu Rev Biochem. 2012;81:145-66
pubmed: 22663078
Cold Spring Harb Perspect Biol. 2017 May 1;9(5):null
pubmed: 28062564
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6686-91
pubmed: 11390996
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1619-1624
pubmed: 28143937
J Biol Chem. 2002 Feb 15;277(7):4883-91
pubmed: 11729207
Nat Rev Genet. 2013 Dec;14(12):880-93
pubmed: 24217315
Mol Biol Cell. 2005 Apr;16(4):1987-2002
pubmed: 15689496
Clin Cancer Res. 2014 Mar 15;20(6):1531-41
pubmed: 24449823
Cancer Lett. 2018 Apr 1;418:185-195
pubmed: 29339211
Cold Spring Harb Perspect Biol. 2016 May 02;8(5):
pubmed: 27141051
Biochim Biophys Acta. 2014 Jul;1843(7):1414-26
pubmed: 24703882
Mol Cell. 2003 Apr;11(4):915-26
pubmed: 12718878
Nat Commun. 2016 Jun 22;7:12047
pubmed: 27329220
Autophagy. 2015;11(12):2172-83
pubmed: 26565952
EMBO J. 2003 Oct 15;22(20):5323-35
pubmed: 14532106
Proc Natl Acad Sci U S A. 2015 May 5;112(18):5785-90
pubmed: 25902512
EMBO J. 2004 Oct 13;23(20):4061-71
pubmed: 15385962
Stem Cells. 2013 Dec;31(12):2827-32
pubmed: 24022994